Soybean aphid biotype 1 genome: Insights into the invasive biology and adaptive evolution of a major agricultural pest
Soybean aphid biotype 1 genome: Insights into the invasive biology and adaptive evolution of a major agricultural pest
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DOI：
10.1016/j.ibmb.2020.103334
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发表时间：
2020-05-01
影响因子：
3.8
通讯作者：
Zhan, Shuai
中科院分区：
文献类型：
--
作者：
Giordano, Rosanna;Donthu, Ravi Kiran;Zhan, Shuai
大豆麦长管蚜是世界主要农作物大豆的重要害虫。我们组装了AP的从头基因组序列。甘氨酸生物型1，来自该物种入侵北美后不久建立的培养体系。占AP的20.4%。甘氨酸蛋白质组是复制的。这些并列基因富含基因本体论(GO)类别，主要与细胞凋亡有关，这可能是对植物化学和其他环境胁迫的一种适应。其中大约三分之一的基因在其他蚜虫中表现出平行的重复。但美联社。与其关系最近的棉属物种，这些重复基因的数量最少。采用Illumina GoldenGate方法对2380个SNPs进行分析，以确定AP在世界范围内的种群结构。甘氨酸。中国和韩国蚜虫距离北美最近。中国可能是其他亚洲蚜虫种群的起源。与北美的蚜虫关系最远的是澳大利亚。AP的多样性。自它到来以来，北美的甘氨酸随着时间的推移而减少。AP的遗传多样性。在2001年首次发现甘氨酸后不久至2012年，北美人口中的甘氨酸似乎与地理位置无关。然而，在明尼苏达州(MN)、爱荷华州(IA)和威斯康星州(WI)更接近高密度鼠李林的大豆RAG试验品种上收集的蚜虫，似乎比在俄亥俄州(OH)、北达科他州(ND)和南达科他州(SD)采样的蚜虫具有更高的定植抗性大豆植株的能力。来自前几个州的样本具有高F-ST值和频率的SNP等位基因，这些等位基因与铁代谢相关的基因重叠，铁代谢是一种关键的代谢途径，可能受到RAG相关大豆植株反应的影响。美联社。甘氨酸生物型1基因组将为未来分析蚜虫毒力和抗药性机制提供必要的信息，并有助于不同自然历史和寄主植物范围的蚜虫之间的比较分析。
The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae) is a serious pest of the soybean plant, Glycine max, a major world-wide agricultural crop. We assembled a de novo genome sequence of Ap. glycines Biotype 1, from a culture established shortly after this species invaded North America. 20.4% of the Ap. glycines proteome is duplicated. These in-paralogs are enriched with Gene Ontology (GO) categories mostly related to apoptosis, a possible adaptation to plant chemistry and other environmental stressors. Approximately one-third of these genes show parallel duplication in other aphids. But Ap. gossypii, its closest related species, has the lowest number of these duplicated genes. An Illumina GoldenGate assay of 2380 SNPs was used to determine the world-wide population structure of Ap. Glycines. China and South Korean aphids are the closest to those in North America. China is the likely origin of other Asian aphid populations. The most distantly related aphids to those in North America are from Australia. The diversity of Ap. glycines in North America has decreased over time since its arrival. The genetic diversity of Ap. glycines North American population sampled shortly after its first detection in 2001 up to 2012 does not appear to correlate with geography. However, aphids collected on soybean Rag experimental varieties in Minnesota (MN), Iowa (IA), and Wisconsin (WI), closer to high density Rhamnus cathartica stands, appear to have higher capacity to colonize resistant soybean plants than aphids sampled in Ohio (OH), North Dakota (ND), and South Dakota (SD). Samples from the former states have SNP alleles with high F-ST values and frequencies, that overlap with genes involved in iron metabolism, a crucial metabolic pathway that may be affected by the Rag-associated soybean plant response. The Ap. glycines Biotype 1 genome will provide needed information for future analyses of mechanisms of aphid virulence and pesticide resistance as well as facilitate comparative analyses between aphids with differing natural history and host plant range.