Structures, functions and molecular evolution of the penta-EF-hand Ca2+-binding proteins
Structures, functions and molecular evolution of the penta-EF-hand Ca2+-binding proteins
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DOI：
10.1016/s1570-9639(02)00444-2
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发表时间：
2002-11-04
影响因子：
3.2
通讯作者：
Shibata, H
中科院分区：
文献类型：
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作者：
Maki, M;Kitaura, Y;Shibata, H
Penta-EF-hand (PEF) 蛋白由 Ca2+ 结合蛋白家族组成，具有五个重复的 EF-hand 基序。在八个α螺旋（α1-α8）中，α4和α7分别连接EF2-EIF3和EF4-EF5。除了 EF 手区域的结构相似性外，PEF 蛋白家族成员还具有共同特征：(i) 通过不配对的 C 端 EF5 进行二聚化，(ii) 拥有疏水性 Gly/Pro 丰富的 N 端结构域，以及 (.)(iii) Ca2+ 依赖性易位至膜。根据氨基酸序列的比较，哺乳动物PEF蛋白分为两类：第一类PEF蛋白（ALG-2和peflin）和第二类PEF蛋白（Ca2+依赖性蛋白酶钙蛋白酶亚家族成员，sorcin和grancalcin）。 I 组基因也在低等动物、植物、真菌和原生生物中被发现。最近对特定相互作用蛋白的发现已开始逐渐揭示非催化哺乳动物 PEF 蛋白的功能。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Penta-EF-hand (PEF) proteins comprise a family of Ca2+-binding proteins that have five repetitive EF-hand motifs. Among the eight alpha-helices (alpha1-alpha8), alpha4 and alpha7 link EF2-EIF3 and EF4-EF5, respectively. In addition to the structural similarities in the EF-hand regions, the PEF protein family members have common features: (i) dimerization through unpaired C-terminal EF5s, (ii) possession of hydrophobic Gly/ Pro-rich N-terminal domains, and (.)(iii) Ca2+-dependent translocation to membranes. Based on comparison of amino acid sequences, mammalian PEF proteins are classified into two groups: Group I PEF proteins (ALG-2 and peflin) and Group II PEF proteins (Ca2+- dependent protease calpain subfamily members, sorcin and grancalcin). The Group I genes have also been found in lower animals, plants, fungi and protists. Recent findings of specific interacting proteins have started to gradually unveil the functions of the noncatalytic mammalian PEF proteins. (C) 2002 Elsevier Science B.V. All fights reserved.