Neuroprotective Effects of Flax Lignan Against NMDA-Induced Neurotoxicity In Vitro
Neuroprotective Effects of Flax Lignan Against NMDA-Induced Neurotoxicity In Vitro
复制标题
亚麻木酚素对 NMDA 诱导神经毒性的体外神经保护作用
DOI：
10.1111/cns.12003
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发表时间：
2012-01-01
影响因子：
5.5
通讯作者：
Zhao, Ming-Gao
中科院分区：
文献类型：
--
作者：
Li, Xu-Bo;Yang, Zhao-Xu;Zhao, Ming-Gao
Aims: Flax Lignan (FLL), a chemical widespread within the plant and animal kingdoms, has antioxidant, antiinfectious, and antitumor activities. However, little is known about the effects of FLL on the central nervous system (CNS). Methods: The neuroprotective actions of FLL against N-methyl-d-aspartate (NMDA) are investigated in primary cultured cortical neurons by MTT assay. The expression levels of proteins related to apoptosis and GluN2-containing receptor were detected by Western blot analysis. Intracellular Ca2+ was measured under a confocal laser scanning microscope. Results: After challenged with 100 mu M NMDA for 30 min, loss of cell viability and excessive apoptotic cell death were observed in cultured cortical neurons. FLL protected the neurons against the NMDA-induced cell loss in a concentration-dependent manner. FLL also significantly inhibited the neuronal apoptosis induced by NMDA exposure through reversing intracellular concentration of Ca2+ overload and balancing of Bcl-2 and Bax expression. Furthermore, FLL significantly reversed the upregulation of GluN2B-containing NMDA receptors by exposure to NMDA, but did not affect the expression of GluN2A-containing NMDA receptor. Conclusions: These findings suggest that FLL protects cortical neurons by inhibiting the expression of GluN2B-containing NMDA receptor and regulating the Bcl-2 family.
