The reversible developmental unipotency of germ cells in chicken
The reversible developmental unipotency of germ cells in chicken
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DOI：
10.1530/rep-09-0265
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发表时间：
2010-01-01
期刊：
影响因子：
3.8
通讯作者：
Han, Jae Yong
中科院分区：
文献类型：
--
作者：
Jung, Jin Gyoung;Lee, Young Mok;Han, Jae Yong
我们最近开发了双模态生殖系嵌合体生产方法，通过将原始生殖细胞（PGCs）或胚胎生殖细胞（EGCs）转移到胚胎中，并通过将精原干细胞（SSC）或生殖系干细胞（GSC）移植到成年睾丸中。本研究利用我们建立的生殖系嵌合体生产系统研究鸡生殖细胞的可逆发育单能性。首先，我们将新鲜分离的成体睾丸精原干细胞或体外培养的GSCs移植到X期和14 - 16期胚胎中，发现这些移植的精原干细胞/GSCs可以迁移到受体胚胎的性腺中。在接受SSC或GSC的527个胚胎中，有135个产生了孵化。在17个性成熟的雄性（35.3%）中，通过测交分析确定了6个为生殖系嵌合体，平均生殖系传播效率为1.3%。第二，将从胚胎性腺中分离的生殖细胞PGCs/EGCs移植到成年睾丸中。EGC移植诱导生殖系传递，而PGC移植没有。EGC睾丸移植（EGCs至成年睾丸）的生殖系传递效率是SSC/GSC胚胎移植（睾丸生殖细胞至胚胎）的12.5倍（16.3vs1.3%）。总之，鸡的生殖细胞从不同的发展阶段可以（去）分化成配子，即使生殖细胞发育时钟设置后或提前。利用生殖细胞可逆单能性可能会提高生殖细胞介导的生殖系传播的效率。生殖（2010年）139 113 - 119
We recently developed bimodal germline chimera production approaches by transfer of primordial germ cells (PGCs) or embryonic germ cells (EGCs) into embryos and by transplantation of spermatogonial stem cells (SSCs) or germline stem cells (GSCs) into adult testes. This study was undertaken to investigate the reversible developmental unipotency of chicken germ cells using our established germline chimera production systems. First, we transferred freshly isolated SSCs from adult testis or in vitro cultured GSCs into stage X and stage 14-16 embryos, and we found that these transferred SSCs/GSCs could migrate to the recipient embryonic gonads. Of the 527 embryos that received SSCs or GSCs, 135 yielded hatchlings. Of 17 sexually mature males (35.3%), six were confirmed as germline chimeras through testcross analysis resulting in an average germline transmission efficiency of 1.3%. Second, PGCs/EGCs, germ cells isolated from embryonic gonads were transplanted into adult testes. The EGC transplantation induced germline transmission, whereas the PGC transplantation did not. The germline transmission efficiency was 12.5 fold higher (16.3 vs 1.3%) in EGC transplantation into testis (EGCs to adult testis) than that in SSC/GSC transfer into embryos (testicular germ cells to embryo stage). In conclusion, chicken germ cells from different developmental stages can (de)differentiate into gametes even after the germ cell developmental clock is set back or ahead. Use of germ cell reversible unipotency might improve the efficiency of germ cell-mediated germline transmission. Reproduction (2010) 139 113-119