Variation of 423G>T in the Agouti Gene Exon 4 in Indigenous Chinese Goat Breeds
Variation of 423G>T in the Agouti Gene Exon 4 in Indigenous Chinese Goat Breeds
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DOI：
10.1007/s10528-008-9192-4
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发表时间：
2008-09
影响因子：
2.4
通讯作者：
Chun-juan Tang;R. Zhou;Xianglong Li;Jing-wen Zhao;Lan-hui Li;Fu-Jun Feng;Dong-feng Li;Jiantiao Wang;Xiu-li Guo;Jing-Fen Keng
中科院分区：
文献类型：
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作者：
Chun-juan Tang;R. Zhou;Xianglong Li;Jing-wen Zhao;Lan-hui Li;Fu-Jun Feng;Dong-feng Li;Jiantiao Wang;Xiu-li Guo;Jing-Fen Keng
agoutigene在家畜色素合成中起着重要作用。在对山羊agouttigene 4外显子片段（178 bp）进行扩增和测序后，发现了banii识别的423G b> T的反转。采用PCR-RFLP方法对12个中国本土山羊品种和1个南非进口山羊品种（布尔山羊）的677个个体进行遗传效应和多样性分析。检测到2个等位基因T和G， 3个基因型TT、TG和GG。等位基因T在大多数山羊品种中频率较高，结合毛色表型，被认为是导致黑色表型的原因，或者与山羊的致病位点有关。结果还表明，在中国黑色毛色山羊品种中，423G>T的遗传多样性较低。13个山羊群体的遗传分化率为0.2023。基于423G>T位点的种群聚类与被毛颜色的变化基本一致。
TheAgoutigene plays an important role in pigment synthesis in domestic animals. A transversion of 423G>T recognized byBanII was found after a fragment (178 bp) of the goatAgoutigene exon 4 was amplified and sequenced. To investigate its genetic effect and diversity, 677 individuals from 12 indigenous Chinese goat breeds and one imported goat breed from South Africa (Boer goat) were analyzed by PCR-RFLP. Two alleles, T and G, and three genotypes, TT, TG, and GG, were detected. Allele T had a higher frequency in most goat breeds and, combined with the coat color phenotype, is believed to be responsible for the black phenotype or to be linked with the causative site in the goat. The results also indicate that the 423G>T transversion showed lower genetic diversity in goat breeds with black coat color in China. Genetic differentiation among the 13 goat populations was 0.2023. The clustering of populations based on the 423G>T site was basically consistent with the variation of coat color.