MicroRNA signature in response to nutrient restriction and re-feeding in fast skeletal muscle of grass carp (Ctenopharyngodon idella).
MicroRNA signature in response to nutrient restriction and re-feeding in fast skeletal muscle of grass carp (Ctenopharyngodon idella).
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DOI：
10.13918/j.issn.2095-8137.2014.5.404
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发表时间：
2014-09
期刊：
影响因子：
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通讯作者：
Xin Zhu;W. Chu;Ping Wu;Tan Yi;Tao Chen;Jianshe Zhang
中科院分区：
文献类型：
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作者：
Xin Zhu;W. Chu;Ping Wu;Tan Yi;Tao Chen;Jianshe Zhang
草鱼（Ctenopharyngodon idella）是我国重要的养殖鱼类之一。越来越多的证据表明microRNAs（miRNAs）可能是草鱼骨骼肌的关键调控因子，但目前对肌源性miRNAs的身份及其在骨骼肌合成代谢中的作用的认识还很有限。在本研究中，我们选择了8个先前报道的与肌肉生长相关的miRNAs用于禁食-再喂养研究。我们研究了禁食一周的草鱼幼鱼在饱食一餐后8种miRNAs表达的变化，发现7种miRNAs在再投喂后1或3 h内表达显著上调，表明它们可能是参与调节鱼类骨骼肌生长的快速反应信号系统的有希望的候选miRNAs。
The grass carp (Ctenopharyngodon idella) is one of the most important cultivated fish species in China. Mounting evidences suggests that microRNAs (miRNAs) may be key regulators of skeletal muscle among the grass carp, but the knowledge of the identity of myogenic miRNAs and role of miRNAs during skeletal muscle anabolic state remains limited. In the present study, we choose 8 miRNAs previously reported to act as muscle growth-related miRNAs for fasting-refeeding research. We investigated postprandial changes in the expression of 8 miRNAs following a single satiating meal in grass carp juveniles who had been fasting for one week and found that 7 miRNAs were sharply up-regulated within 1 or 3 h after refeeding, suggesting that they may be promising candidate miRNAs involved in a fast-response signaling system that regulates fish skeletal muscle growth.