Striking phenotypic and functional differences in lamina propria lymphocytes from the large and small intestine of mice
Striking phenotypic and functional differences in lamina propria lymphocytes from the large and small intestine of mice
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DOI：
10.1016/j.lfs.2004.08.042
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发表时间：
2005-04-29
期刊：
影响因子：
6.1
通讯作者：
Moreno-Fierros, L
中科院分区：
文献类型：
--
作者：
Reséndiz-Albor, AA;Esquivel, R;Moreno-Fierros, L
尽管已知大肠 (LI) 的上皮内 T 淋巴细胞在表型和功能上与小肠 (SI) 的不同，但 LI 和 SI 固有层 (LP) 淋巴细胞群的差异尚未明确确定。在这项工作中，我们发现通过流式细胞术分析的 Balb/c 小鼠的 SI 和 LI LP 淋巴细胞群之间存在显着的表型差异。在LI中，大多数淋巴细胞是B细胞，主要T细胞是TCR-(αβ(+)、CD8(+)。相反，在SI中，大多数T淋巴细胞是表达TCR-αβ(+)的CD4(+)，尽管表达TCR-γδ(+)的比例比U中更高。在T细胞中，SI和LT之间粘附分子和细胞因子的表达也不同。LP T细胞表达TCR-αβ(+)的比例较高。 LI 中的 alpha(4)beta(7) 和 L-选择素含量高于 SI；而 SI 中检测到的表达 alpha(E)beta(7) 的细胞比例高于 LT。LI 的上皮内区室中检测到的表达 L-选择素和 alpha(4)beta(1) 的 T 细胞比例高于 SI，而 SI 中表达 alpha(E)beta(7) 的 T 细胞数量远高于 LT。小肠和大肠上皮内和固有层自发产生 IL-2、IFN γ 和 IL-4 的 T 细胞比例不同，表明不同肠区的淋巴细胞群存在独特的功能特征 (c) 2005 Elsevier Inc. 保留所有权利。
Although intraepithelial T lymphocytes of the large intestine (LI) are known to differ from those of the small intestine (SI) in phenotype and function.. differences in LI and SI lamina propria (LP) lymphocyte populations have not been clearly established. In this work we found striking phenotypic differences between SI and LI LP lymphocyte populations from Balb/c mice analyzed by flow cytometry. In the LI most lymphocytes were B cells and the predominant T cells were TCR-(alpha beta(+), CD8(+). In contrast, in the SI most T lymphocytes were CD4(+) expressing TCR-alpha beta(+), although a higher proportion expressed TCR-gamma delta(+) than in the U. In T cells the expression of adhesion molecules and cytokines was also different between SI and LT. The proportion of LP T cells expressing alpha(4)beta(7) and L-selectin was higher in the LI than in the SI; whereas a greater proportion of cells expressing alpha(E)beta(7) were detected in the SI than in LT. Higher proportions of T cells expressing L-selectin and alpha(4)beta(1) were detected in the intraepithelial compartment of the LI than that of the SI, whereas the number of T cells expressing alpha(E)beta(7) was much higher in the SI than in the LT. The proportion of T cells spontaneously producing IL-2, IFN gamma, and IL-4 at the intraepithelial and lamina propria, in the small and large intestine, was different indicating that distinctive functional features exist in the lymphocyte populations residing at the different intestinal compartments. (c) 2005 Elsevier Inc. All rights reserved.