Wax moth larva (Galleria mellonella): an in vivo model for assessing the efficacy of antistaphylococcal agents
Wax moth larva (Galleria mellonella): an in vivo model for assessing the efficacy of antistaphylococcal agents
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DOI：
10.1093/jac/dkr198
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发表时间：
2011-08-01
影响因子：
5.2
通讯作者：
Coote, Peter J.
中科院分区：
文献类型：
--
作者：
Desbois, Andrew P.;Coote, Peter J.
目的：研究大蜡螟幼虫是否是评价抗金黄色葡萄球菌和耐甲氧西林葡萄球菌药物体内疗效的合适宿主。方法：用递增剂量的金黄色葡萄球菌（MRSA）感染蜡螟幼虫。金黄色葡萄球菌，以研究接种量对幼虫存活的影响。此外，用达托霉素、青霉素或万古霉素处理感染的蜡螟幼虫，以检查这些药物是否对S。结果：随着活菌接种量的增加，金黄色葡萄球菌和MRSA的感染率增加。金黄色葡萄球菌细胞导致更高的幼虫死亡率，但热灭活细菌和无细胞培养物对存活没有不利影响。幼虫死亡率也取决于接种后的孵化温度。幼虫感染S.金黄色葡萄球菌，幼虫存活率提高了抗葡萄球菌抗生素达托霉素或万古霉素。幼虫的存活率随着抗生素剂量的增加而增加。此外，青霉素改善了感染青霉素敏感甲氧西林敏感沙门氏菌的幼虫的存活率。金黄色葡萄球菌（MSSA）菌株，但在感染MRSA（青霉素耐药）的幼虫中以类似剂量无效。达托霉素和万古霉素也是有效的，当给药的幼虫感染bacterios.Conclusions：这是第一次报告证明，抗生素是有效的蜡螟幼虫模型治疗革兰氏阳性菌引起的感染。新的蜡螟幼虫模型是一个有用的初步模型，用于评估候选抗葡萄球菌药物的体内疗效，然后再进行哺乳动物研究，这可能会减少动物实验和费用。
Objectives: To investigate whether the wax moth larva, Galleria mellonella, is a suitable host for assessing the in vivo efficacy of antistaphylococcal agents against Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) infections.Methods: Wax moth larvae were infected with increasing doses of S. aureus to investigate the effect of inoculum size on larval survival. In addition, infected wax moth larvae were treated with daptomycin, penicillin or vancomycin to examine whether these agents were effective against S. aureus and MRSA infections in vivo.Results: Increasing inoculum doses of live S. aureus cells resulted in greater larval mortality, but heat-killed bacteria and cell-free culture filtrates had no detrimental effects on survival. Larval mortality rate also depended on the post-inoculation incubation temperature. After larvae were infected with S. aureus, larval survival was enhanced by administering the antistaphylococcal antibiotics daptomycin or vancomycin. Larval survival increased with increasing doses of the antibiotics. Moreover, penicillin improved survival of larvae infected with a penicillin-susceptible methicillin-susceptible S. aureus (MSSA) strain, but it was ineffective at similar doses in larvae infected with MRSA (penicillin resistant). Daptomycin and vancomycin were also effective when administered to the larvae prior to infection with bacteria.Conclusions: This is the first report to demonstrate that antibiotics are effective in the wax moth larva model for the treatment of infections caused by Gram-positive bacteria. The new wax moth larva model is a useful preliminary model for assessing the in vivo efficacy of candidate antistaphylococcal agents before proceeding to mammalian studies, which may reduce animal experimentation and expense.