Characteristics and origin of myogenic response in isolated mesenteric arterioles.
Characteristics and origin of myogenic response in isolated mesenteric arterioles.
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DOI：
10.1152/ajpheart.1992.263.5.h1486
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发表时间：
1992-11
期刊：
影响因子：
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通讯作者：
D. Sun;E. Messina;G. Kaley;A. Koller
中科院分区：
文献类型：
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作者：
D. Sun;E. Messina;G. Kaley;A. Koller
在无流量条件下研究了离体大鼠肠系膜小动脉对血管内压力变化的反应。分离第一代、第二代、第三代和第四代小动脉并插管。使用图像剪切装置测量并记录血管直径。小动脉（第一代血管除外）产生自发张力，对应于血管内压力的逐步增加（从 20 到 160 mmHg，以 20 mmHg 为步长）。例如，在80毫米汞柱压力下，第一代、第二代、第三代和第四代血管的平均直径分别为286.9+/-5.0、203.4+/-8.2、92.5+/-4.6和35.6+/-4.8微米；通过使用含有乙二醇-双(β-氨基乙基醚)-N,N,N',N'-四乙酸(1 mM)和硝普钠(SNP; 10(-4) M)的无Ca(2+)溶液，这些血管的被动直径为295.6+/-6.3、238.4+/-11.7、120.3+/-3.7，并且分别为 59.4 +/- 3.1 微米，表明压力引起的收缩程度随着世代的增加而增加（分别为 3%、14%、24% 和 43%）。通过空气去除内皮前后小动脉对乙酰胆碱(ACh；10(-6)M)和SNP(10(-7)M)的反应来评估内皮和血管平滑肌的血管活性功能。去除内皮后，取消对 ACh 的扩张，同时保留对 SNP 的扩张。内皮细胞的去除并没有显着改变小动脉直径随血管内压力逐步增加而发生的变化。（摘要截断为 250 字）
Responses to changes in intravascular pressure of isolated rat mesenteric arterioles were investigated under no-flow conditions. First-, second-, third-, and fourth-generation arterioles were isolated and cannulated. Vascular diameters were measured with an image-shearing device and recorded. The arterioles (except for the first-generation vessels) developed spontaneous tone, corresponding to the step increases in intravascular pressure (from 20 to 160 mmHg, by 20-mmHg steps). For example, at 80 mmHg pressure the mean diameters of first-, second-, third-, and fourth-generation vessels were 286.9 +/- 5.0, 203.4 +/- 8.2, 92.5 +/- 4.6, and 35.6 +/- 4.8 microns, respectively; by use of a Ca(2+)-free solution containing ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (1 mM) and sodium nitroprusside (SNP; 10(-4) M) the passive diameters of these vessels were 295.6 +/- 6.3, 238.4 +/- 11.7, 120.3 +/- 3.7, and 59.4 +/- 3.1 microns, respectively, demonstrating that the degree of pressure-induced constriction increased with the increasing order of generations (3, 14, 24, and 43%, respectively). The vasoactive function of endothelium and vascular smooth muscle was assessed by the responses of arterioles to acetylcholine (ACh; 10(-6) M) and SNP (10(-7) M) before and after removal of the endothelium with air. After removal of the endothelium, dilation to ACh was abolished while dilation to SNP was retained. Removal of the endothelium did not significantly alter the changes in the diameter of arterioles in response to step increases in intravascular pressure.(ABSTRACT TRUNCATED AT 250 WORDS)