Sp1 and Sp3 regulate basal transcription of the survivin gene
Sp1 and Sp3 regulate basal transcription of the survivin gene
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Sp1 和 Sp3 调节生存素基因的基础转录
DOI：
10.1016/j.bbrc.2007.02.140
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发表时间：
2007-04-27
影响因子：
3.1
通讯作者：
Qian, GuanXiang
中科院分区：
文献类型：
--
作者：
Xu, Rang;Zhang, Ping;Qian, GuanXiang
Survivin是凋亡抑制蛋白家族的独特成员，在许多癌症中过度表达，被认为在肿瘤发生中起重要作用。本研究克隆并鉴定了survivin基因近端269 bp的启动子，该启动子在HeLa细胞中表现出较强的启动子活性。无tata，富含gc的启动子包含Sp1的7个假定结合位点，其中两个（一个位于-148至-153位置，另一个位于-127至-140位置）在调节基础survivin启动子活性中至关重要。Sp1和Sp3都可以激活survivin启动子，EMSA证实了这一点，用RNAi或米霉素处理HeLa细胞阻断Sp1或Sp3， Sp1或Sp3过表达。我们的研究结果表明Sp1与Sp3共同调控survivin启动子活性。(c) 2007爱思唯尔公司版权所有。
Survivin, a unique member of the inhibitor of apoptosis protein family, is overexpressed in many cancers and considered to play an important role in oncogenesis. In this study, we cloned and identified the proximal 269 bp promoter of survivin gene, which exhibited strong promoter activity in HeLa cells. The TATA-less, GC-rich promoter contains 7 putative binding sites for Sp1, two of which (one at position -148 to -153, the other at position -127 to -140) are essential in regulating basal survivin promoter activity. Not only Sp1 but also Sp3 can activate the survivin promoter, which were proven by EMSA, blocking Sp1 or Sp3 using RNAi or mithramycin treatment of HeLa cells, and overexpression of Sp1 or Sp3. Our results collectively suggest that Sp1 cooperates with Sp3 to regulate survivin promoter activity. (c) 2007 Elsevier Inc. All rights reserved.