Isolation and characterization of lamprey rhodopsin cDNA.
Isolation and characterization of lamprey rhodopsin cDNA.
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七鳃鳗视紫红质 cDNA 的分离和表征。
DOI：
10.1016/0006-291x(91)91537-m
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发表时间：
1991
影响因子：
3.1
通讯作者：
Akira Yasui
中科院分区：
文献类型：
--
作者：
O. Hisatomi;Tatsuo Iwasa;Fumio Tokunaga;Akira Yasui
用寡核苷酸混合物作为引物，通过聚合酶链反应扩增编码七鳃鳗视觉色素的基因组DNA片段。通过分别扩增cDNA两端获得cDNA的完整编码区。推导的编码区氨基酸序列与高等脊椎动物视紫红质的同源性为78 - 82%，而与人类色素的同源性仅为43 - 47%。克隆的DNA似乎是七鳃鳗视紫红质的cDNA，其在“短”感光细胞中表达。
Genomic DNA fragments coding a visual pigment of the lamprey were amplified by polymerase chain reaction, using oligonucleotide mixtures as primers. The complete coding region of the cDNA was obtained by separate amplification of both cDNA ends. The deduced amino acid sequence of the coding region showed 78 – 82 % identity with those of rhodopsins of higher vertebrates, but only 43 – 47 % identity with those of human color pigments. The cloned DNA appears to be the cDNA of a lamprey rhodopsin, which is expressed in the “short” photoreceptor cell.
影响因子：
56.9
作者：
E. Zhukovsky;D. Oprian
通讯作者：
E. Zhukovsky;D. Oprian