Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library.
Peptide substrate identification for yeast Hsp40 Ydj1 by screening the phage display library.
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DOI：
10.1251/bpo90
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发表时间：
2004
影响因子：
6.4
通讯作者：
Sha B
中科院分区：
文献类型：
--
作者：
Li J;Sha B
我们利用噬菌体展示文库筛选和等温滴定口径(ITC)相结合的方法，鉴定了分子伴侣Hsp40 Ydj1的多肽底物。利用7聚噬菌体展示文库对Hsp40Ydj1的多肽底物进行了初步筛选。合成了生物淘洗后的多肽序列，并通过等温滴定计量法对Hsp40 Ydj1的亲和力进行了直接测定。与Ydj1具有可测量亲和力的多肽在底物片段的中间显示丰富的疏水残基。分析了分子伴侣Hsp40的多肽底物专一性。
We have identified a peptide substrate for molecular chaperone Hsp40 Ydj1 by utilizing the combination of phage display library screening and isothemol titration calirimetry (ITC). The initial peptide substrate screening for Hsp40 Ydj1 has been carried out by utilizing a 7-mer phage display library. The peptide sequences from the bio-panning were synthesized and object to the direct affinity measurement for Hsp40 Ydj1 by isothemol titration calirimetry studies. The peptide which has the measurable affinity with Ydj1 shows enriched hydrophobic residues in the middle of the substrate fragment. The peptide substrate specificity for molecular chaperone Hsp40 has been analyzed.