Multifunctional Nanobeads Based on Quantum Dots and Magnetic Nanoparticles: Synthesis and Cancer Cell Targeting and Sorting
Multifunctional Nanobeads Based on Quantum Dots and Magnetic Nanoparticles: Synthesis and Cancer Cell Targeting and Sorting
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DOI：
10.1021/nn102761t
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发表时间：
2011-02-01
期刊：
影响因子：
17.1
通讯作者：
Pellegrino, Teresa
中科院分区：
文献类型：
--
作者：
Di Corato, Riccardo;Bigall, Nadja C.;Pellegrino, Teresa
通过使磁性纳米颗粒、量子点和两亲性聚合物的混合物去稳定化，然后用叶酸分子使珠表面官能化来制备三官能聚合物纳米珠。可以使用乙腈或水作为去稳定溶剂来调节纳米珠内纳米颗粒的分布。所得珠的发光可以通过改变每个磁性纳米颗粒的量子点的比率来调节。将外部磁场（例如0.3T的小静态磁体）施加到磁性荧光纳米珠粒允许珠粒在几个小时内的定量积累，这取决于珠粒的总尺寸。此外，由于叶酸装饰了合成的纳米珠的表面，实现了过表达叶酸受体的癌细胞的特异性靶向。叶酸受体介导的细胞摄取的叶酸功能化的纳米珠被证明通过共聚焦成像和透射电子显微镜表征。用三功能纳米珠进行的细胞分选实验显示，即使当靶细胞以低百分比（高达1%）存在时，也能定量回收靶细胞。
Trifunctional polymer nanobeads are prepared by destabilization of a mixture of magnetic nanoparticles, quantum dots, and an amphiphilic polymer, followed by functionalization of the bead surface with folic acid molecules. The distribution of the nanoparticles within the nanobeads can be tuned using either acetonitrile or water as destabilizing solvent. The luminescence of the resulting beads can be tuned by varying the ratio of quantum dots per magnetic nanoparticles. The application of an external magnetic field (such as a small static magnet of 0.3 T) to the magnetic-fluorescent nanobeads allows the quantitative accumulation of the beads within a few hours depending on the total size of the beads. Furthermore, specific targeting of cancer cells overexpressing folate receptors is achieved thanks to the folic acid decorating the surface of the as-synthesized nanobeads. Folate receptor mediated cellular uptake of the folic acid-functionalized nanobeads is proven via both confocal imaging and transmission electron microscopy characterization. Cell sorting experiments performed with trifunctional nanobeads show quantitative recovering of targeted cells even when they are present at low percentage (up to 1%).