MULTIPLE FORMS OF PROLACTIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID ARE SPECIFICALLY EXPRESSED AND REGULATED IN MURINE TISSUES AND THE MAMMARY CELL LINE-HC11
MULTIPLE FORMS OF PROLACTIN RECEPTOR MESSENGER-RIBONUCLEIC-ACID ARE SPECIFICALLY EXPRESSED AND REGULATED IN MURINE TISSUES AND THE MAMMARY CELL LINE-HC11
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DOI：
10.1210/en.130.3.1108
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发表时间：
1992-03-01
期刊：
影响因子：
4.8
通讯作者：
BALL, RK
中科院分区：
文献类型：
--
作者：
BUCK, K;VANEK, M;BALL, RK
研究小鼠组织中PRL受体mRNA的表达。共发现7种不同大小的PRL受体转录本，其表达模式具有组织特异性。PRL受体蛋白以大约300和600个氨基酸的同种型存在，其在胞质结构域的序列和长度上不同。特异于较低分子量形式的受体的探针与主要在肝脏和肾脏中表达的1.4、2.4、3.5和4.2 kb的转录物杂交。在受体的三种亚型中，PR-3高表达，PR-2弱表达，PR-1检测不到。探针特异性较高的摩尔重量受体形式检测到9和10 kb的成绩单，表达最强烈的卵巢，乳腺和肾脏。另外一个8.3 kb的转录本在肾脏中表达。PRL反应的小鼠乳腺上皮细胞系HC 11只表达9-和10-kb受体mRNA，乳腺也是如此。两种PRL受体形式的转录本在妊娠和哺乳期间以组织特异性方式独立调节。小受体形式在肝脏中的表达在妊娠期间增加7倍，在哺乳期间减少。其在肾脏中的表达保持恒定。大受体形式的表达增加了2.5倍，在哺乳期的肾脏，但在乳腺中保持不变。在卵巢中，大受体形式的表达在用FSH和hCG诱导超排卵后可升高4倍。因此，在小鼠中，至少有两种形式的PRL受体在不同组织中独立调节。
The expression of PRL receptor mRNA in mouse tissues was studied. Seven PRL receptor transcripts of different sizes were found. The pattern of expression was tissue specific. The PRL receptor protein exists in isoforms of approximately 300 and 600 amino acids, which differ in the sequence and length of the cytoplasmic domain. Probes specific for the lower mol wt forms of the receptor hybridized to transcripts of 1.4, 2.4, 3.5, and 4.2 kilobases (kb), which were predominantly expressed in the liver and kidney. Among the three isoforms of the small form of the receptor, PR-3 was highly expressed, PR-2 was weakly expressed, and PR-1 was undetectable. Probes specific for the higher mol wt receptor form detected transcripts of 9 and 10 kb, expressed most strongly in the ovary, mammary gland, and kidney. An additional 8.3-kb transcript was expressed in the kidney. The PRL-responsive mouse mammary epithelial cell line HC11 expressed only the 9- and 10-kb receptor mRNAs, as did the mammary gland. The transcripts for the two PRL receptor forms were independently regulated during pregnancy and lactation in a tissue-specific manner. The expression of the small receptor form in the liver increased 7-fold during pregnancy and decreased during lactation. Its expression in the kidney remained constant. Expression of the large receptor form increased 2.5-fold in the kidney during lactation, but remained constant in the mammary gland. In the ovary the expression of the large receptor form could be elevated 4-fold after induction of hyperovulation with FSH and hCG. Thus, in the mouse there are at least two forms of the PRL receptor regulated independently in different tissues.