Integration host factor is required for the activation of developmentally regulated genes in Caulobacter.
Integration host factor is required for the activation of developmentally regulated genes in Caulobacter.
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柄杆菌中发育调控基因的激活需要整合宿主因子。
DOI：
10.1101/gad.4.9.1494
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发表时间：
1990
影响因子：
10.5
通讯作者：
Shapiro,L
中科院分区：
文献类型：
--
作者：
Gober,JW;Shapiro,L
新月柄杆菌中的几个时间控制的鞭毛基因需要σ 54启动子和上游转录激活位点。我们在这里证明，在这些基因中的一些，一个AT丰富的区域包含一个整合宿主因子（IHF）的共识结合位点之间的激活剂和启动子，该区域结合IHF在体外。钩操纵子的IHF结合区的突变分析表明，一个完整的IHF结合位点是必要的在体内转录。相邻的趋异启动子也具有结合IHF的IHF共有序列。IHF和增强子位点位于该启动子中转录起始位点的3'端。我们假设，IHF介导的方式类似于为大肠杆菌DNA重组产生的核小体样结构的不同的启动子区域之间的高阶结构的形成，这种高阶结构调节转录。
Several temporally controlled flagellar genes in Caulobacter crescentus require a sigma 54 promoter and upstream sites for transcription activation. We demonstrate here that in some of these genes, an AT-rich region containing an integration host factor (IHF) consensus binding site lies between the activator and the promoter, and that this region binds IHF in vitro. Analysis of mutations in the IHF-binding region of the hook operon demonstrated that an intact IHF-binding site is necessary for transcription in vivo. An adjacent and divergent promoter also has an IHF consensus sequence that binds IHF. The IHF and enhancer sites are 3' to the transcription start site in this promoter. We postulate that IHF mediates the formation of a higher order structure between the divergent promoter regions in a manner analogous to the nucleosome-like structure generated for lambda-Escherichia coli DNA recombination and that this higher order structure modulates transcription.