Hydrogen-rich saline reverses oxidative stress, cognitive impairment, and mortality in rats submitted to sepsis by cecal ligation and puncture
Hydrogen-rich saline reverses oxidative stress, cognitive impairment, and mortality in rats submitted to sepsis by cecal ligation and puncture
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DOI：
10.1016/j.jss.2012.01.041
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发表时间：
2012-11-01
影响因子：
2.2
通讯作者：
Wang, Jian
中科院分区：
文献类型：
--
作者：
Zhou, Jun;Chen, Ye;Wang, Jian
背景：脓毒症与高发病率和死亡率相关，幸存者可表现为认知功能障碍。本研究旨在探讨富氢盐水（HRS）对脓毒症大鼠脑氧化应激、认知功能障碍和死亡率的影响。方法：采用盲肠结扎穿刺法建立脓毒症大鼠模型。术前10分钟腹腔注射生理盐水或HRS （2.5 mL/kg或10 mL/kg）。记录大鼠存活率，并采用Morris水迷宫测试认知功能。通过观察海马中活性氧、丙二醛水平和超氧化物歧化酶活性来评估氧化应激水平。检测caspase 3水平，检测细胞凋亡。采用苏木精-伊红染色和末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口端标记法观察海马组织病理变化。结果：盲肠结扎和穿刺导致生存率低，脑损伤和认知功能障碍的证据。海马活性氧和丙二醛水平显著升高，超氧化物歧化酶活性显著降低。HRS以剂量依赖性的方式逆转了这些变化。结论：这些发现表明，HRS可以减轻盲肠结扎和穿刺引起的大鼠脓毒症的后果，至少部分是通过抑制氧化应激。(C) 2012爱思唯尔公司版权所有。
Background: Sepsis is associated with high morbidity and mortality, and survivors can present with cognitive dysfunction. The present study was performed to investigate the effects of hydrogen-rich saline (HRS) on oxidative stress in the brain, cognitive dysfunction, and mortality in a rat model of sepsis.Methods: A rat model of sepsis was induced by cecal ligation and puncture. Physiologic saline or HRS was administered intraperitoneally (2.5 mL/kg or 10 mL/kg) 10 min before the operation. The survival rate was recorded, and cognitive function was tested using the Morris water maze. The reactive oxygen species and malondialdehyde levels and superoxide dismutase activity in the hippocampus were observed to evaluate the oxidative stress levels. The caspase 3 levels were measured to detect apoptosis. The histopathologic changes in the hippocampus were evaluated by hematoxylin-eosin staining and the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay.Results: Cecal ligation and puncture resulted in a poor survival rate, evidence of brain injury, and cognitive dysfunction. The hippocampal reactive oxygen species and malondialdehyde levels increased significantly, and superoxide dismutase activity decreased significantly. HRS reversed these changes in a dose-dependent manner.Conclusions: These findings indicate that HRS could attenuate the consequences of sepsis induced by cecal ligation and puncture in rats, at least in part, by the inhibition of oxidative stress. (C) 2012 Elsevier Inc. All rights reserved.