MCM2 and Ki-67 expression in human lung adenocarcinoma: Prognostic implications
MCM2 and Ki-67 expression in human lung adenocarcinoma: Prognostic implications
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DOI：
10.1159/000078673
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发表时间：
2004-01-01
期刊：
影响因子：
5
通讯作者：
Ito, H
中科院分区：
文献类型：
--
作者：
Hashimoto, K;Araki, K;Ito, H
目的：检测微小染色体维持蛋白2（MCM 2）、Ki-67和p53在肺腺癌中的表达，分析其病理生物学意义。研究方法：我们在6个人肺腺癌细胞系和145例手术切除的腺癌中进行了Western印迹分析和免疫组化，以检查MCM 2的表达。将肿瘤细胞中MCM 2、Ki-67和p53的标记指数（LI; %）与临床病理特征和总生存率进行比较。结果：在所有检测的细胞系中均检测到MCM 2蛋白，并出现特异性条带。MCM 2 LI与性别、组织学类型、分化程度、病理分期及Ki-67、p53 LI均显著相关（p < 0.05）。122例非单纯性细支气管肺泡癌中MCM 2和Ki-67的LI值明显高于23例单纯性细支气管肺泡癌（P < 0.01），前者预后差于后者（P < 0.01）。性别、病理分期、MCM 2和/或Ki-67的高LI是独立的预后因素（p < 0.05）。结论：肺腺癌（非单纯性细支气管肺泡癌）患者MCM 2和/或Ki-67的高LI提示预后不良。版权所有（C）2004 S. Karger AG，巴塞尔。
Objective: The expressions of minichromosome maintenance protein 2 (MCM2), Ki-67, and p53 were examined to analyze their pathobiological significance in human lung adenocarcinomas. Methods: We performed Western blot analysis in six human lung adenocarcinoma cell lines and immunohistochemistry in 145 surgically removed adenocarcinomas to examine the MCM2 expression. Labeling indices (LIs; %) of MCM2, Ki-67, and p53 in the tumor cells were compared with clinicopathological profiles and overall survival rates. Results: MCM2 protein was detected in all cell lines examined, with specific bands. MCM2 LIs were significantly correlated with sex, histological type, differentiation, pathological stage, and LIs of Ki-67 and p53 (p < 0.05). Significantly higher LIs of MCM2 and Ki-67 were noted in the 122 non-pure bronchioloalveolar carcinomas than in the 23 pure bronchioloalveolar carcinomas (p < 0.01), and the prognosis was poorer in the former than in the latter (p < 0.01). Sex, pathological stage, and high LIs of MCM2 and/or Ki-67 were independent prognostic factors (p < 0.05). Conclusion: High LIs of MCM2 and/or Ki-67 suggest a poor prognosis in patients with lung adenocarcinoma (non-pure bronchioloalveolar carcinoma). Copyright (C) 2004 S. Karger AG, Basel.