Interaction of Cd-hyperaccumulator Solanum nigrum L. and functional endophyte Pseudomonas sp Lk9 on soil heavy metals uptake
Interaction of Cd-hyperaccumulator Solanum nigrum L. and functional endophyte Pseudomonas sp Lk9 on soil heavy metals uptake
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DOI：
10.1016/j.soilbio.2013.10.021
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发表时间：
2014-01-01
影响因子：
9.7
通讯作者：
Liu, Chengbin
中科院分区：
文献类型：
--
作者：
Chen, Liang;Luo, Shenglian;Liu, Chengbin
生物强化是一种有助于从污染土壤中植物提取重金属的有前景的方法，而生物强化辅助植物提取的发展需要了解植物与接种物之间相互作用所涉及的机制。在本研究中，进行了一项盆栽试验，以评估能够产生生物表面活性剂、铁载体和有机酸的细菌内生菌假单胞菌属Lk9对在多金属污染土壤中生长的镉超富集植物龙葵的生长和金属吸收的影响。结果表明，接种Lk9能够改善土壤铁和磷矿物质营养供应，提高土壤重金属有效性，并影响宿主介导的低分子量有机酸分泌，从而使龙葵地上部分干生物量显著增加14%，地上部分积累的镉总量增加46.6%、锌增加16.4%、铜增加16.0%，相较于未接种的对照。植物提取评估显示，接种Lk9提高了镉的生物富集系数（28.9%）以及所有金属的植物提取率（镉、锌和铜分别为17.4%、48.6%和104.6%），而转运系数在接种Lk9（镉、锌和铜分别为3.30、0.50和0.40）和未接种对照（镉、锌和铜分别为2.95、0.53和0.42）之间差异可忽略不计。还发现与未接种Lk9的龙葵相比，龙葵与Lk9的共生显著使土壤微生物量碳增加39.2%，酸性磷酸酶活性增加28.6%。本研究将为重金属污染土壤的生物强化辅助植物提取提供新的见解。（C）2013爱思唯尔有限公司。保留所有权利。
Bioaugmentation is a promising method for assisting phytoextraction of heavy metals from contaminated soil, and the development of bioaugmentation-assisted phytoextraction requires the understanding of the mechanism involved in the interaction between plants and inocula. In this study, a pot study was conducted to evaluate the effect of bacterial endophyte Pseudomonas sp. Lk9 which can produce biosurfactants, siderophores and organic acids on the growth and metal uptake of Cd-hyperaccumulator Solanum nigrum L. growing in multi-metal-contaminated soil. The results revealed that Lk9 inoculation could improve soil Fe and P mineral nutrition supplies, enhance soil heavy metal availability, and affect host-mediated low-molecular-weight organic acids secretion, thereby significantly increasing S. nigrum shoot dry biomass by 14% and the total of Cd by 46.6%, Zn by 16.4% and Cu by 16.0% accumulated in aerial parts, compared to those of non-inoculated control. The assessment of phytoextraction showed that Lk9 inoculation elevated the bioaccumulation factor of Cd (28.9%) and phytoextraction rates of all metals (17.4%, 48.6% and 104.6% for Cd, Zn and Cu, respectively), while the translocation factors had negligible difference between Lk9 inoculation (3.30, 0.50 and 0.40 for Cd, Zn and Cu, respectively) and non-inoculated control (2.95, 0.53 and 0.42 for Cd, Zn and Cu, respectively). It was also found that the symbiotic association between S. nigrum and Lk9 significantly increased the soil microbial biomass C by 39.2% and acid phosphatase activity by 28.6% compared to those in S. nigrum without Lk9. This study would provide a new insight into the bioaugmentation-assisted phytoextraction of heavy metal-contaminated soils. (C) 2013 Elsevier Ltd. All rights reserved.