Variable channel expression in identified single and electrically coupled neurons in different animals
Variable channel expression in identified single and electrically coupled neurons in different animals
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DOI：
10.1038/nn1639
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发表时间：
2006-03-01
影响因子：
25
通讯作者：
Marder, E
中科院分区：
文献类型：
--
作者：
Schulz, DJ;Goaillard, JM;Marder, E
通常认为，相同细胞类型的所有神经元都具有相同的内在特性，无论是在动物体内还是在动物之间。我们利用螃蟹口胃神经节中的大尺寸和小数量的明确可识别的神经元来测试通道mRNA表达和膜电流（在电压钳实验中测量）水平上的这一假设。在外侧幽门（LP）神经元中，我们看到测量电流与Shal和BK-KCa mRNA（分别编码Shal家族电压门控钾通道和大电导钙激活钾通道）丰度之间存在强相关性。我们还看到三种钾电流及其mRNA表达的两到四倍动物间变异性。在两个电耦合的幽门扩张（PD）神经元的通道表达的测量显示出显着的动物间的差异，但IH（编码超极化激活，内向电流通道）和Shal mRNA的拷贝数在两个PD神经元从同一螃蟹是相似的，这表明一些电流的调节可能是共享的电耦合神经元。
It is often assumed that all neurons of the same cell type have identical intrinsic properties, both within an animal and between animals. We exploited the large size and small number of unambiguously identifiable neurons in the crab stomatogastric ganglion to test this assumption at the level of channel mRNA expression and membrane currents (measured in voltage-clamp experiments). In lateral pyloric (LP) neurons, we saw strong correlations between measured current and the abundance of Shal and BK-KCa mRNAs (encoding the Shal-family voltage-gated potassium channel and large-conductance calcium-activated potassium channel, respectively). We also saw two- to fourfold interanimal variability for three potassium currents and their mRNA expression. Measurements of channel expression in the two electrically coupled pyloric dilator (PD) neurons showed significant interanimal variability, but copy numbers for IH (encoding the hyperpolarization-activated, inward-current channel) and Shal mRNA in the two PD neurons from the same crab were similar, suggesting that the regulation of some currents may be shared in electrically coupled neurons.