Charge inversion in DNA-amphiphile complexes: possible application to gene therapy
Charge inversion in DNA-amphiphile complexes: possible application to gene therapy
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DOI：
10.1016/s0378-4371(99)00409-4
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发表时间：
1999-12-01
期刊：
影响因子：
3.3
通讯作者：
Barbosa, MC
中科院分区：
文献类型：
--
作者：
Kuhn, PS;Levin, Y;Barbosa, MC
我们研究了DNA和阳离子两亲分子之间的复合物形成。当两亲物加入到含有DNA的溶液中时，发现表面活性剂与DNA分子的协同结合，这种结合转变发生在特定的两亲物密度下，这强烈地依赖于盐的浓度和表面活性剂分子的疏水性。我们发现，对于足够疏水的两亲物，复合物的电荷中和，甚至电荷反转是可能的。这在基因治疗的应用中是特别重要的，对于基因治疗，将特定碱基序列功能性地递送到活细胞中仍然是一个突出的问题。原则上，电荷反转可以使DNA-表面活性剂复合物接近带负电荷的细胞膜，从而允许转染发生。(C)1999 Elsevier Science B. V.保留所有权利。
We study complex formation between the DNA and cationic amphiphilic molecules. As the amphiphile is added to the solution containing DNA, a cooperative binding of surfactants to the DNA molecules is found. This binding transition occurs at a specific density of amphiphile, which is strongly dependent on the concentration of the salt and on the hydrophobicity of the surfactant molecules. We find that for amphiphiles which are sufficiently hydrophobic, a charge neutralization, or even charge inversion of the complex is possible. This is of particular importance in applications to gene therapy, for which the functional delivery of specific base sequence into living cells remains an outstanding problem. The charge inversion could, in principle, allow the DNA-surfactant complexes to approach the negatively charged cell membranes permitting the transfection to take place. (C) 1999 Elsevier Science B.V. All rights reserved.