Comparative analysis of the complete genome sequences of Helicoverpa zea and Helicoverpa armigera single-nucleocapsid nucleopolyhedroviruses
Comparative analysis of the complete genome sequences of Helicoverpa zea and Helicoverpa armigera single-nucleocapsid nucleopolyhedroviruses
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DOI：
10.1099/0022-1317-83-3-673
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发表时间：
2002-03-01
影响因子：
3.8
通讯作者：
Vlak, JM
中科院分区：
文献类型：
--
作者：
Chen, XW;Zhang, WJ;Vlak, JM
测定了玉米棉铃虫（Helicoverpazea）单核衣壳核多角体病毒（Helicoverpanucleopolyhedrovirus，HzSNPV）的全序列（130869 bp），并与棉铃虫（Helicoverpaarmigera，Ha）SNPV的全序列进行了比较。这两个基因组在它们的核苷酸（97%同一性）和氨基酸（99%同一性）序列上非常相似。编码区比非编码区保守得多。在HzSNPV/HaSNPV中，63个开放阅读框（ORF）在所有已测序的杆状病毒中存在，比其他ORF保守得多。与HaSNPV相比，HzSNPV具有四个额外的小ORF，其中一个（Hz 42）处于正确的转录背景中。HzSNPV和HaSNPV之间的主要差异在于同源区（hrs）和杆状病毒重复ORF（bro基因）的序列和组织。HzSNPV和HaSNPV hrs之间的序列同一性在90%（hr 1）至几乎100%（hr 5）的范围内，并且hrs的不同在于存在/不存在一个或多个A型和/或B型重复。HzSNPV的三个bro基因与HaSNPV中的基因显著不同，并且可能在祖先的过去独立获得。序列数据强烈表明HzSNPV和HaSNPV是相同病毒物种的变体，这一结论得到了物理和生物学数据的支持。
The complete nucleotide sequence of Helicoverpa zea single-nucleocapsid nucleopolyhedrovirus (HzSNPV) has been determined (130869 bp) and compared to the nucleotide sequence of Helicoverpa armigera (Ha) SNPV. These two genomes are very similar in their nucleotide (97% identity) and amino acid (99% identity) sequences. The coding regions are much more conserved than the non-coding regions. In HzSNPV/HaSNPV, the 63 open reading frames (ORFs) present in all baculoviruses sequenced so far are much more conserved than other ORFs. HzSNPV has four additional small ORFs compared with HaSNPV, one of these (Hz42) being in a correct transcriptional context. The major differences between HzSNPV and HaSNPV are found in the sequence and organization of the homologous regions (hrs) and the baculovirus repeat ORFs (bro genes). The sequence identity between the HzSNPV and HaSNPV hrs ranges from 90% (hr1) to almost 100% (hr5) and the hrs differ in the presence/absence of one or more type A and/or B repeats. The three HzSNPV bro genes differ significantly from those in HaSNPV and may have been acquired independently in the ancestral past. The sequence data suggest strongly that HzSNPV and HaSNPV are variants of the same virus species, a conclusion that is supported by the physical and biological data.