A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles.
A Novel Conductive Poly(3,4-ethylenedioxythiophene)-BSA Film for the Construction of a Durable HRP Biosensor Modified with NanoAu Particles.
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新型导电聚（3,4-乙撑二氧噻吩）-BSA 薄膜，用于构建纳米金颗粒修饰的耐用 HRP 生物传感器
DOI：
10.3390/s16030374
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发表时间：
2016-03-15
期刊：
影响因子：
--
通讯作者：
Gu Y
中科院分区：
文献类型：
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作者：
Xu F;Ren S;Gu Y
在这项研究中，我们研究了牛血清白蛋白（BSA）的贡献，电化学合成的聚（3，4-乙撑二氧噻吩）（PEDOT）膜的耐久性铂（Pt）电极。该电极能够有效地吸附纳米Au粒子（AuNPs），形成均匀的布局，进而能够吸附辣根过氧化物酶（HRP），构建功能性HRP/AuNPs/PEDOT（BSA）/Pt生物传感器。采用循环伏安法对过氧化氢的测定结果进行了评价。结果表明，阴极电流与H_2O_2浓度之间存在良好的线性关系。此外，在检测溶液中添加氧化形式的烟酰胺腺嘌呤二核苷酸或NAD+作为电子转移介质可以显著提高检测灵敏度约35.5%。目前的生物传感器的主要优点是其耐用性，灵敏度，可靠性和生物相容性。
In this study, we have investigated the contribution of bovine serum albumin (BSA) to the durability of the electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) film on a platinum (Pt) electrode. The electrode was capable to effectively adsorb the nano Au particles (AuNPs) to form a uniform layout, which was then able to immobilize the horseradish peroxidase (HRP) to construct a functional HRP/AuNPs/PEDOT(BSA)/Pt biosensor. Cyclic voltammetry was employed to evaluate the performance of the biosensor through the measurement of hydrogen peroxide. Our results revealed a satisfied linear correlation between the cathodic current and the concentration of H2O2. Furthermore, the addition of oxidized form of nicotinamide adenine dinucleotide, or NAD+, as the electron transfer mediator in the detection solution could dramatically enhance the sensitivity of detection by about 35.5%. The main advantages of the current biosensor are its durability, sensitivity, reliability, and biocompatibility.