Lead, zinc, and copper levels in intraocular brain tissue grafts, brain, and blood of lead-exposed rats.
Lead, zinc, and copper levels in intraocular brain tissue grafts, brain, and blood of lead-exposed rats.
复制标题
铅暴露大鼠的眼内脑组织移植物、大脑和血液中的铅、锌和铜水平。
DOI：
10.1016/0041-008x(81)90327-6
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发表时间：
1981
影响因子：
3.8
通讯作者：
Olson,L
中科院分区：
文献类型：
--
作者：
Björklund,H;Lind,B;Piscator,M;Hoffer,B;Olson,L
成年雌性SD大鼠暴露于不同浓度的醋酸铅不同时间。接触0.125%、0.25%、0.25%、0.5%、1%和2%醋酸铅6周后，脑铅和血铅水平升高，与饮用水中铅浓度高度相关。当动物暴露在1%的醋酸铅中不同的时间长度时，铅进入大脑的速度在第一天就明显延迟。然而，如果动物暴露在更长的时间内，大脑铅水平的增加速度快于血液水平。将胎儿大脑皮质移植到暴露于1%醋酸铅或醋酸钠的宿主动物的前房内。移植后6周，铅暴露组的铅浓度为31.6 mg/kg干重，而醋酸钠对照组为6.4 mg/kg干重。然而，两组移植物的铅水平大约是宿主大脑相应区域大脑皮层铅含量的五倍。此外，与原位皮质相比，移植物中的锌和铜水平也更高。结合以前关于动物和人类铅暴露的报道，这些数据表明，接受眼内脑移植的成年大鼠口服铅可以产生与临床铅中毒问题有关的血液和脑水平。
Adult female Sprague-Dawley rats were exposed to various concentrations of lead acetate for different lengths of time. Six weeks exposure to lead acetate at concentrations of 0, 0.125, 0.25, 0.5, 1, and 2% in the drinking water, gave rise to brain and blood lead levels that were highly correlated with the lead concentration in the drinking water. When animals were exposed to 1% lead acetate for different lengths of time, an apparent delay in the rate of lead transport into the brain was seen during the first day. However, if animals were exposed for longer time periods, brain lead levels increased faster than did the blood levels. Pieces of fetal cortex cerebri were grafted to the anterior eye chamber of host animals exposed to either 1% lead acetate or to sodium acetate. Six weeks after grafting, the lead concentration in the lead-exposed grafts were 31.6 mg/kg dry wt, as compared to 6.4 mg/kg dry wt in sodium acetate control grafts. However, grafts from both groups had lead levels that were approximately five times higher than in cerebral cortex “punches” from corresponding areas of the host brains. Furthermore, zinc and copper levels were also higher in the grafts as compared to punches of in situ cortex. Taken together with previous reports on animal and human lead exposure, these data indicate that oral lead intake in adult rats bearing intraocular brain grafts yields blood and brain levels which are physiologically relevant to problems of clinical lead toxicity.