Cancer prevention and therapy with kiwifruit in Chinese folklore medicine:: a study of kiwifruit extracts
Cancer prevention and therapy with kiwifruit in Chinese folklore medicine:: a study of kiwifruit extracts
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DOI：
10.1016/s0378-8741(02)00125-3
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发表时间：
2002-08-01
影响因子：
5.4
通讯作者：
Molnár, J
中科院分区：
文献类型：
--
作者：
Motohashi, N;Shirataki, Y;Molnár, J
分别用己烷、丙酮、甲醇和70%甲醇提取猕猴桃金果，用硅胶和ODS柱层析进行分离，测定其各种生物活性。H1、H2（己烷提取物）、A1、A2（丙酮提取物）和M2（甲醇提取物）5个组分对人口腔肿瘤细胞系具有选择性细胞毒活性，且对人牙龈成纤维细胞的敏感性高于人牙龈成纤维细胞。70%甲醇提取物的亲水组分[70M3, 70M4, 70M5]表现出较高的抗hiv活性、自由基生成和O-2（-）清除活性。70%甲醇提取物[70M0, 70M1, 70M2, 70M3, 70M4]的抑菌活性普遍低于亲脂性组分（己烷、丙酮、甲醇提取物），但各组分均未表现出特异性抑菌作用。所有馏分对幽门螺杆菌均无活性。这些结果表明，金猕猴桃提取物中含有多种有价值的生物活性物质，可以相互分离。(C) 2002爱思唯尔科学爱尔兰有限公司版权所有。
Kiwi gold fruits were extracted successively with hexane, acetone, methanol and 70% methanol, and further fractionated by silica gel and ODS column chromatographies for the assays of various biological activities. Five fractions H1, H2 (hexane extract), A1, A2 (acetone extract) and M2 (methanol extract) showed selective cytotoxic activity against human oral tumor cell lines, which was more sensitive than human gingival fibroblasts. More hydrophilic fractions [70M3, 70M4, 70M5] of 70% methanol extract displayed higher anti-HIV activity, radical generation and O-2(-) scavenging activity. The antibacterial activity of 70% methanol extracts [70M0, 70M1, 70M2, 70M3, 70M4] was generally lower than that of more lipophilic fractions (hexane, acetone, methanol extracts), although each fraction did not show any specific antimicrobial action. All fractions were inactive against Helicobacter pylori. These results demonstrate that gold kiwifruit extracts contain valuable, various bioactive materials, which can be separated with each other. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.