Label-free metabolic biomarkers for assessing valve interstitial cell calcific progression
Label-free metabolic biomarkers for assessing valve interstitial cell calcific progression
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用于评估瓣膜间质细胞钙化进展的无标记代谢生物标志物
DOI：
10.1038/s41598-020-66960-4
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发表时间：
2020
影响因子：
4.6
通讯作者：
Balachandran, Kartik
中科院分区：
文献类型：
--
作者：
Tandon, Ishita;Kolenc, Olivia I.;Cross, Delaney;Vargas, Isaac;Johns, Shelby;Quinn, Kyle P.;Balachandran, Kartik
钙化性主动脉瓣疾病（CAVD）是最常见的瓣膜疾病，唯一可用的治疗策略是外科瓣膜置换术。CAVD的早期检测技术将有利于预防、缓解和替代治疗策略的发展。双光子激发荧光（TPEF）显微镜是一种无标记的非破坏性成像技术，已被证明与癌症和伤口愈合中细胞分化和表型变化的多种标志物相关。在这里，我们展示了特定的TPEF标志物，即光学氧化还原比和线粒体分形维数，与成骨分化过程中主动脉瓣间质细胞（VIC）发生的结构，功能和表型变化相关。线粒体的光氧化还原比和分形维数进行了评估，并与VIC的基因表达和核形态。在早期成骨分化过程中，VIC的光学氧化还原率降低，并与CAVD进展的生物标志物相关。分形维数与结构和成骨标志物以及核形态的措施。我们的研究表明，TPEF成像标志物，特别是光学氧化还原比和线粒体分形维数，可以作为一种工具，用于评估早期CAVD的进展，在体外。
Calcific aortic valve disease (CAVD) is the most common form of valve disease where the only available treatment strategy is surgical valve replacement. Technologies for the early detection of CAVD would benefit the development of prevention, mitigation and alternate therapeutic strategies. Two-photon excited fluorescence (TPEF) microscopy is a label-free, non-destructive imaging technique that has been shown to correlate with multiple markers for cellular differentiation and phenotypic changes in cancer and wound healing. Here we show how specific TPEF markers, namely, the optical redox ratio and mitochondrial fractal dimension, correlate with structural, functional and phenotypic changes occurring in the aortic valve interstitial cells (VICs) during osteogenic differentiation. The optical redox ratio, and fractal dimension of mitochondria were assessed and correlated with gene expression and nuclear morphology of VICs. The optical redox ratio decreased for VICs during early osteogenic differentiation and correlated with biological markers for CAVD progression. Fractal dimension correlated with structural and osteogenic markers as well as measures of nuclear morphology. Our study suggests that TPEF imaging markers, specifically the optical redox ratio and mitochondrial fractal dimension, can be potentially used as a tool for assessing early CAVD progressionin vitro.
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影响因子：
14
作者：
Farrar, Emily J.;Pramil, Varsha;Richards, Jennifer M.;Mosher, Christopher Z.;Butcher, Jonathan T.
通讯作者：
Butcher, Jonathan T.
使用 Na3PO4 增强主动脉瓣钙化的体外动物模型。
DOI：
--
发表时间：
2016
期刊：
International Journal of Cardiovascular Research
影响因子：
--
作者：
D. Lerman;S. Prasad;N. Alotti
通讯作者：
N. Alotti
瓣膜间质细胞形状调节细胞收缩力，与细胞表型无关。
影响因子：
2.4
作者：
Ishita Tandon;A. Razavi;Prashanth Ravishankar;Addison M Walker;Nasya M. Sturdivant;N. Lam;J. Wolchok;Kartik Balachandran
通讯作者：
Kartik Balachandran
影响因子：
9.7
作者：
Duan, Bin;Hockaday, Laura A.;Kapetanovic, Edi;Kang, Kevin H.;Butcher, Jonathan T.
通讯作者：
Butcher, Jonathan T.
影响因子：
3.9
作者：
Kamel Pi;Q. X.;K. J. Grande-Allen;Peter I. Kamel;X. Qu;Andrew M. Geiszler;Deepak Nagrath;R. Harmancey;H. Taegtmeyer
通讯作者：
Kamel Pi;Q. X.;K. J. Grande-Allen;Peter I. Kamel;X. Qu;Andrew M. Geiszler;Deepak Nagrath;R. Harmancey;H. Taegtmeyer