Reactive species responsible for the inactivation of feline calicivirus by a two-dimensional array of integrated coaxial microhollow dielectric barrier discharges in air
Reactive species responsible for the inactivation of feline calicivirus by a two-dimensional array of integrated coaxial microhollow dielectric barrier discharges in air
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DOI：
10.1002/ppap.201700119
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发表时间：
2018
影响因子：
3.5
通讯作者：
G. Nayak;H. Aboubakr;S. Goyal;P. Bruggeman
中科院分区：
文献类型：
--
作者：
G. Nayak;H. Aboubakr;S. Goyal;P. Bruggeman
低温等离子体用于生物净化和灭菌已受到越来越多的关注。在这项研究中，在常压干燥空气中产生的二维集成同轴微空心微放电阵列用于处理已知浓度的猫杯状病毒（FCV）污染的金属表面（气相）和溶液（液相）。FCV是人类诺如病毒的替代品，诺如病毒是导致人类急性肠胃炎暴发的原因。研究和比较了两种处理方法的去污效果以及导致病毒灭活的主要化学途径。研究发现，在干燥空气中气相处理的生物样品的湿度需要在3 min内实现FCV滴度降低bb50 log10。发现气相FCV失活是由于臭氧（O3）和活性氮（RNS）（最有可能是NOx）的共同作用。液相FCV失活机制是ph依赖性的，主要是由于RNS，最有可能是酸化亚硝酸盐。O3对悬浮在溶液中的FCV的影响可以忽略不计。先前在间歇式反应器中进行的研究表明，由于高浓度NxOy的臭氧中毒，通过O3和RNS的失活途径是相互排斥的。本研究采用了一种流动系统，避免了活性物质的积累，并允许NOx和O3在本研究中使用的气体停留时间内共存，从而产生了这些特定的失活途径。
The use of low-temperature plasmas for bio-decontamination and sterilization has been gaining increased attention. In this study, a two-dimensional array of integrated coaxial microhollow micro-discharges generated in dry air at atmospheric pressure is used to treat metal surfaces (gas-phase) and solution (liquid-phase) contaminated with a known concentration of feline calicivirus (FCV). FCV acts as a surrogate for human norovirus, which is responsible for causing outbreaks of acute gastroenteritis in humans. The decontamination efficacy as well as the primary chemical pathways leading to virus inactivation in both the treatments are studied and compared. It is found that the humidity of the bio-sample for gas-phase treatment in dry air is required to achieve >5 log10 reduction in FCV titer within 3 min. The gas-phase FCV inactivation is found to be due to a combination of ozone (O3) and reactive nitrogen species (RNS), most likely NOx. The liquid-phase FCV inactivation mechanism is pH-dependent and is primarily due to RNS, most likely acidified nitrites. O3 has a negligible effect on FCV suspended in solution. Previous studies performed in a batch reactor have shown that the inactivation pathways through O3 and RNS are mutually exclusive due to ozone poisoning at high NxOy concentrations. The present study employs a flow-through system which avoids accumulation of reactive species and allows for the coexistence of NOx and O3 for the gas residence times used in this study, giving rise to these specific inactivation pathways.