Rapid labeling of mitochondrial lipids by labeled orthophosphate and adenosine triphosphate.
Rapid labeling of mitochondrial lipids by labeled orthophosphate and adenosine triphosphate.
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通过标记的正磷酸盐和三磷酸腺苷快速标记线粒体脂质。
DOI：
10.1016/s0021-9258(18)93586-6
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
B. Agranoff
中科院分区：
文献类型：
--
作者：
A. Hajra;E. Seguin;B. Agranoff
研究了与 32 Pi 或 γ-32 P-ATP 一起孵育的豚鼠肝线粒体的快速标记脂质。通过使用氧化磷酸化抑制剂，32Pi 被转化为标记的 ATP，这是标记脂质的更直接的前体。苍术苷在对外加 ATP 的氧化磷酸化或脂质标记几乎没有影响的条件下，可阻断内源 ATP 对脂质的磷酸化。通过色谱法鉴定出两种放射性脂质为磷酸磷脂酰肌醇和磷脂酸。研究了未知的第三种脂质的特性。通过向培养介质中添加羟胺来选择性地阻止其形成。据推测，脂质是通过线粒体部分中的激酶和磷酸酶的作用来标记的。
Rapidly labeled lipids of guinea pig liver mitochondria incubated with32Pior γ-32P-ATP were studied. With the use of inhibitors of oxidative phosphorylation, it was shown that32Piis converted to labeled ATP, which is the more direct precursor of labeled lipid. Atractyloside blocks phosphorylation of lipid by endogenous ATP under conditions in which it has little effect on oxidative phosphorylation or lipid labeling by externally added ATP. Two radioactive lipids were identified by chromatography as phosphatidylinositol phosphate and phosphatidic acid. The properties of an unknown third lipid were studied. Its formation was selectively blocked by the addition of hydroxylamine to the incubation medium. It is postulated that the lipids are labeled by the action of kinases and phosphatases in the mitochondrial fraction.