Effect of mesenchymal stem cells on inhibiting airway remodeling and airway inflammation in chronic asthma
Effect of mesenchymal stem cells on inhibiting airway remodeling and airway inflammation in chronic asthma
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DOI：
10.1002/jcb.24501
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发表时间：
2013-07-01
影响因子：
4
通讯作者：
Chen, Ruohua
中科院分区：
文献类型：
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作者：
Ge, Xiahui;Bai, Chong;Chen, Ruohua
已有研究证明骨髓间充质干细胞（BMSCs）可以通过其免疫调节特性改善多种免疫介导的疾病。在本研究中，我们观察了骨髓间充质干细胞对慢性哮喘小鼠气道重塑和气道炎症的影响。将48只雌性BALB/c小鼠随机分为PBS组、BMSCs治疗组、BMSCs对照组和哮喘组。采用酶联免疫吸附法检测血清和支气管肺泡灌洗液中细胞因子和免疫球蛋白水平。流式细胞术检测CD 4 + CD 25+调节性T细胞的数量，苏木精-伊红染色、免疫荧光染色、Schiff酸染色和Masson染色检测CD 4 + CD 25+调节性T细胞的形态。我们发现哮喘小鼠的气道重塑和气道炎症明显。哮喘组IL-12水平较低，IL-13、IL-4、OVA特异性IgG 1、IgE和IgG 2a水平较高，CD 4 + CD 25+调节性T细胞数量较少。但BMSCs移植可显著降低哮喘患者的气道炎症和气道重塑，降低IL-4、OVA特异性IgE和OVA特异性IgG 1水平，升高IL-12水平和CD 4 + CD 25+调节性T细胞数量（P
Previous studies proved that bone marrow-derived mesenchymal stem cells (BMSCs) could improve a variety of immune-mediated disease by its immunomodulatory properties. In this study, we investigated the effect on airway remodeling and airway inflammation by administrating BMSCs in chronic asthmatic mice. Forty-eight female BALB/c mice were randomly distributed into PBS group, BMSCs treatment group, BMSCs control group, and asthmatic group. The levels of cytokine and immunoglobulin in serum and bronchoalveolar lavage fluid were detected by enzyme-linked immunosorbent assay. The number of CD4+CD25+regulatory T cells and morphometric analysis was determined by flow cytometry, hematoxylin-eosin, immunofluorescence staining, periodic-acid Schiff, and masson staining, respectively. We found that airway remodeling and airway inflammation were evident in asthmatic mice. Moreover, low level of IL-12 and high levels of IL-13, IL-4, OVA-specific IgG1, IgE, and IgG2a and the fewer number of CD4+CD25+regulatory T cells were present in asthmatic group. However, transplantation of BMSCs significantly decreased airway inflammation and airway remodeling and level of IL-4, OVA-specific IgE, and OVA-specific IgG1, but elevated level of IL-12 and the number of CD4+CD25+regulatory T cells in asthma (P