Human Bladder Smooth Muscle Cell Damage in Disturbed Oxygen Tension
Human Bladder Smooth Muscle Cell Damage in Disturbed Oxygen Tension
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DOI：
10.1016/j.urology.2011.06.034
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发表时间：
2011-10-01
期刊：
影响因子：
2.1
通讯作者：
Siroky, Mike B.
中科院分区：
文献类型：
--
作者：
Azadzoi, Kazem M.;Yalla, Subbarao V.;Siroky, Mike B.
目的研究人膀胱平滑肌细胞对氧分压紊乱的反应。临床研究表明，膀胱缺血和下尿路症状在老年patients.MATERIALS和方法之间的密切相关性汇合培养的人膀胱平滑肌细胞正常氧，连续缺氧，和氧化应激（缺氧/复氧）条件下使用计算机oxycycler系统孵育。48小时后，细胞样品收集和处理的氧化损伤的荧光评估，抗氧化能力的酶免疫分析，和transmittingelectronmicroscopic.Results脂质过氧化被发现在细胞缺氧和氧化应激，而蛋白质氧化是明显的氧化应激。细胞抗氧化能力在氧化应激中下降，但在缺氧中保持不变。氧化产物只存在于细胞氧化应激中，而亚硝化产物在缺氧和氧化应激条件下均增加。48小时的缺氧和氧化应激对细胞衰老没有影响。缺氧时细胞膜增厚变形，线粒体肿胀，内质网增大。细胞氧化应激时，细胞膜部分丢失，细胞膜小窝增多，线粒体肿胀，嵴降解，内质网破裂，溶酶体增多。对缺氧的反应与细胞生存信号一致，以科普缺氧。氧化应激的变化表明亚细胞成分的广泛损伤和退化。低氧血症和氧化损伤可能是氧分压紊乱的膀胱平滑肌变性的重要机制。泌尿学78：967.e9-967.e15，2011年。爱思唯尔公司出版
OBJECTIVE To characterize human bladder smooth muscle cell reactions to disturbed oxygen tension. Clinical studies have shown a close correlation between bladder ischemia and lower urinary tract symptoms in elderly patients.MATERIALS AND METHODS Confluent cultured human bladder smooth muscle cells were incubated under normoxia, continuous hypoxia, and oxidative stress (hypoxia/reoxygenation) conditions using a computerized oxycycler system. After 48 hours, cell samples were collected and processed for fluorometric assessment of oxidative injury, enzyme immunoassay of antioxidant capacity, and transmission electron microscopy.RESULTS Lipid peroxidation was found in cell hypoxia and oxidative stress, whereas protein oxidation was evident in oxidative stress only. Cell antioxidant capacity decreased in oxidative stress but remained unchanged in hypoxia. Oxidative products were present in cell oxidative stress only, whereas nitrosative products increased in both hypoxia and oxidative stress conditions. Forty-eight hours of hypoxia and oxidative stress had no effect on cell senescence. Thickened deformed cell membrane, swollen mitochondria, and enlarged endoplasmic reticulum (ER) were found in cell hypoxia. Partially lost cell membrane with increased caveolae, swollen mitochondria with degraded cristae, splintered ER, and increased lysosomes were evident in cell oxidative stress.CONCLUSION Human bladder smooth muscle cells are highly reactive to nonconforming oxygen tension. Reactions to hypoxia are consistent with cell survival signaling to cope with lack of oxygen. Changes in oxidative stress indicate extensive damage and deterioration of the subcellular elements. Hypoxic and oxidative damage may be an important mechanism of smooth muscle degeneration in bladder conditions with disturbed oxygen tension. UROLOGY 78: 967.e9-967.e15, 2011. Published by Elsevier Inc.