NKX3.1 Expression in Salivary Gland "Intraductal" Papillary Mucinous Neoplasm: A Low-Grade Subtype of Salivary Gland Mucinous Adenocarcinoma.
NKX3.1 Expression in Salivary Gland "Intraductal" Papillary Mucinous Neoplasm: A Low-Grade Subtype of Salivary Gland Mucinous Adenocarcinoma.
复制标题
NKX3.1 在唾液腺“导管内”乳头状粘液肿瘤中的表达：唾液腺粘液腺癌的低级别亚型。
DOI：
10.1007/s12105-022-01471-4
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Faquin WC.
中科院分区：
文献类型：
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作者：
Nakaguro M;Sadow PM;Hu R;Hattori H;Kuwabara K;Tsuzuki T;Urano M;Nagao T;Faquin WC.
涎腺导管内乳头状黏液性肿瘤（salivary gland intra-ductal papillary mucinous neoplasm，SG IPMN）是一种新近发现的以黏液分泌细胞乳头状囊状增生为特征的肿瘤。由于重叠的组织学特征和克隆性AKT1p.E17K变异，SG IPMN被认为是粘液腺癌的前体或低级别亚型。NKX3.1是位于染色体8 p上的肿瘤抑制基因，并且是已知的前列腺上皮和口腔内唾液腺粘液腺泡细胞的免疫组化标记物。MethodsWe检索了12个SG IPMN病例，并进行了组织学和遗传学分析。鉴于SG IPMN与粘液腺泡细胞的关联，我们还研究了NKX3.1作为该肿瘤实体的标志物的性能。ResultsDiffuse和强烈的NKX3.1表达在所有SG IPMN的情况下（12/12，100%），以及在正常的粘液腺泡细胞中观察到。相反，粘液表皮样癌和胰腺IPMN病例以及正常浆液性腺泡细胞对NKX3.1呈阴性。在遗传学上，12例病例中有11例（92%）携带AKT1p.E17K变异体。在另一例中检测到一种新的PTEN移码缺失（p.G36Dfs*18）。6例（50%）SG IPMN的组织学特征中至少有一种提示恶性肿瘤，如细胞增生严重、核分裂活跃、Ki-67增殖指数高、淋巴管浸润和淋巴结转移。结论SG IPMN是一种低度恶性的粘液腺癌亚型，可能来源于小唾液腺的粘液腺泡细胞。
BackgroundSalivary gland intraductal papillary mucinous neoplasm (SG IPMN) is a recently proposed entity characterized by a papillary-cystic proliferation of mucin-producing cells. Because of overlapping histologic features and a clonalAKT1p.E17K variant, SG IPMN has been presumed to be a precursor or a low-grade subtype of mucinous adenocarcinoma. NKX3.1 is a tumor suppressor gene located on chromosome 8p and is a known immunohistochemical marker of prostate epithelium and mucinous acinar cells of the intraoral salivary glands.MethodsWe retrieved 12 SG IPMN cases, and performed histologic and genetic analysis. Given the association of SG IPMN with mucinous acinar cells, we also investigated the performance of NKX3.1 as a marker of this tumor entity.ResultsDiffuse and strong NKX3.1 expression was observed in all SG IPMN cases (12/12, 100%) as well as in normal mucinous acinar cells. In contrast, mucoepidermoid carcinoma and pancreatic IPMN cases as well as normal serous acinar cells were negative for NKX3.1. Genetically, 11 of 12 cases (92%) harbored anAKT1p.E17K variant. A novelPTENframeshift deletion (p.G36Dfs*18) was detected in the other single case. At least one of the histologic features implying malignant tumors, such as severe cellular atypia, brisk mitotic activity, high Ki-67 proliferating index, lymphovascular invasion, and lymph node metastasis, was detected in 6 SG IPMN cases (50%).ConclusionThe findings suggest that SG IPMN is a low-grade subtype of mucinous adenocarcinoma which may be derived from mucinous acinar cells of the minor salivary gland.