Impact of New Camera Technologies on Discoveries in Cell Biology.
Impact of New Camera Technologies on Discoveries in Cell Biology.
复制标题
新相机技术对细胞生物学发现的影响。
DOI：
10.1086/689587
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Vale,RonaldD
中科院分区：
文献类型：
--
作者：
Stuurman,Nico;Vale,RonaldD
New technologies can make previously invisible phenomena visible. Nowhere is this more obvious than in the field of light microscopy. Beginning with the observation of “animalcules” by Antonie van Leeuwenhoek, when he figured out how to achieve high magnification by shaping lenses, microscopy has advanced to this day by a continued march of discoveries driven by technical innovations. Recent advances in single-molecule-based technologies have achieved unprecedented resolution, and were the basis of the Nobel prize in Chemistry in 2014. In this article, we focus on developments in camera technologies and associated image processing that have been a major driver of technical innovations in light microscopy. We describe five types of developments in camera technology: video-based analog contrast enhancement, charge-coupled devices (CCDs), intensified sensors, electron multiplying gain, and scientific complementary metal-oxide-semiconductor cameras, which, together, have had major impacts in light microscopy.
