Genetic control of macromolecular synthesis during development of an ascidian: Ascidia nigra.
Genetic control of macromolecular synthesis during development of an ascidian: Ascidia nigra.
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海鞘发育过程中大分子合成的遗传控制：黑海鞘。
DOI：
10.1002/jez.1401640309
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
Kirby D. Smith
中科院分区：
文献类型：
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作者：
Kirby D. Smith
在被囊发育的早期，有两个蛋白质快速合成的时期。第一次开始于受精后不久，一直持续到大约四个细胞期，第二次开始于原肠胚。只有蛋白质合成速度的第二次增加被放线菌素-D抑制。除了最初的高合成速率外，在整个早期发育过程中都有一些蛋白质合成，这对放线菌素-D的处理不敏感。因此，囊状胚胎在发育过程中表现出两种基因组控制。有人认为，其中一个在合成新的信使RNA的水平上起作用，另一个在已有的、稳定的信使RNA翻译水平上起作用。这种调节机制的二分法类似于海胆发育过程中发生的情况。
研究还表明，在海鞘胚胎的四个细胞期左右，RNA的合成首先开始，并且这种合成对放线菌素-D处理敏感。据推测，这种早期的RNA合成解释了镶嵌和调节发育之间的差异。
During early tunicate development, there are two periods of rapid protein synthesis. The first begins shortly after fertilization and continues until about the four cell stage, the second begins at gastrulation. Only the second increase in the rate of protein synthesis is inhibited by actinomycin-D. In addition to the initial high rate of synthesis there is some protein synthesis throughout early development which is insensitive to treatment with actinomycin-D. The tunicate embryo thus shows two kinds of genomic control during development. It is suggested that one of them operates at the level of the synthesis of new messenger RNA, and the other at the level of translation of already existing, stable, messenger RNA. This dichotomy of regulatory mechanisms is similar to that occurring during sea urchin development.
It is also shown that RNA synthesis first begins around the four cell stage in the Ascidian embryo and that this synthesis is sensitive to treatment with actinomycin-D. It is postulated that this early synthesis of RNA accounts for the difference between mosaic and regulative development.