Reversible Mannosylation as a Covalent Binding Adjuvant Enhances Immune Responses for Porcine Circovirus Type 2 Vaccine
Reversible Mannosylation as a Covalent Binding Adjuvant Enhances Immune Responses for Porcine Circovirus Type 2 Vaccine
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DOI：
10.1021/acsomega.8b02264
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发表时间：
2018-12
期刊：
影响因子：
4.1
通讯作者：
Yuanzi Wu;Chao Yan;Jianan He;Wenli Xiong;Shuigen Wu;Shutao Liu;Zhen Cai
中科院分区：
文献类型：
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作者：
Yuanzi Wu;Chao Yan;Jianan He;Wenli Xiong;Shuigen Wu;Shutao Liu;Zhen Cai
猪圆环病毒2型（PCV 2）衣壳蛋白是治疗断奶仔猪多系统衰竭综合征的理想亚单位疫苗候选物。在本研究中，研究了甘露聚糖介导的PCV 2 Δ Cap 42 -233蛋白靶向抗原呈递细胞（APC）以开发PCV 2疫苗。甘露聚糖通过酸敏感的Schiff碱反应连接到PCV 2 Δ Cap 42 -233蛋白上。甘露糖基化蛋白具有靶向APC上的甘露糖受体的能力以及在溶酶体的酸性条件下控制释放抗原的能力。最后，评价甘露糖基化PCV 2 Δ Cap 42 -233蛋白在小鼠中的免疫应答。甘露糖基化蛋白具有刺激体液免疫应答和增强免疫力的能力。因此，酸敏感和APC靶向甘露糖基化PCV 2 Δ Cap 42 -233蛋白代表了作为针对猪圆环病毒的有效疫苗的潜在商业应用的有希望的候选者。
Capsid protein of porcine circovirus type 2 (PCV2) is an ideal subunit vaccine candidate for the postweaning multisystemic wasting syndrome. In this study, mannan-mediated targeting of PCV2ΔCap42-233 protein to antigen presenting cells (APCs) was investigated for the development of PCV2 vaccine. Mannan was attached to PCV2ΔCap42-233 protein via an acid sensitive Schiff base reaction. The mannosylated protein was endowed with the capacity to target the mannose receptor on APCs as well as the ability of controlled release of the antigen in the acidic condition of the lysosome. Finally, the immune response of mannosylated PCV2ΔCap42-233 protein in mice was evaluated. The mannosylated protein exhibited the ability to stimulate humoral immune response and enhance the immunity. Thus, acid-sensitive and APCs-targeting mannosylated PCV2ΔCap42-233 protein represents a promising candidate for the potential commercial application as an efficient vaccine against porcine circovirus.