A protein key to plant virus transmission at the tip of the insect vector stylet
A protein key to plant virus transmission at the tip of the insect vector stylet
复制标题
DOI：
10.1073/pnas.0706608104
复制
发表时间：
2007-11-13
影响因子：
11.1
通讯作者：
Blanc, Stephane
中科院分区：
文献类型：
--
作者：
Uzest, Marilyne;Gargani, Daniel;Blanc, Stephane
数百种植物病毒，其中许多具有重要的经济意义，通过非循环载体传播（通过将病毒体附着到载体口器上并通过随后的释放接种）进行传播，但载体内的病毒受体仍然难以捉摸。在这里，我们报告的证据的存在，精确的位置，和化学性质的第一个受体的非循环病毒，花椰菜花叶病毒，在其昆虫载体。电子显微镜显示病毒样颗粒在一个以前未描述的解剖区在最尖端的蚜虫上颌管针。一种新的在体外相互作用测定的特点结合花椰菜花叶病毒蛋白P2（介导病毒载体相互作用）解剖蚜虫针。P2-GFP融合体专门标记位于共同食物/唾液管底部床中的微小表皮结构域。未观察到与非载体物种的探针结合，并且废除P2传递活性的点突变与受损的探针结合相关。新的受体似乎是一个非糖基化的蛋白质深深嵌入在几丁质基质。对这种昆虫受体分子的深入了解将开始打开这一科学领域的主要黑匣子，并可能导致对抗病毒传播的新策略。
Hundreds of species of plant viruses, many of them economically important, are transmitted by noncirculative vector transmission (acquisition by attachment of virions to vector mouthparts and inoculation by subsequent release), but virus receptors within the vector remain elusive. Here we report evidence for the existence, precise location, and chemical nature of the first receptor for a noncirculative virus, cauliflower mosaic virus, in its insect vector. Electron microscopy revealed virus-like particles in a previously undescribed anatomical zone at the extreme tip of the aphid maxillary stylets. A novel in vitro interaction assay characterized binding of cauliflower mosaic virus protein P2 (which mediates virus-vector interaction) to dissected aphid stylets. A P2-GFP fusion exclusively labeled a tiny cuticular domain located in the bottom-bed of the common food/salivary duct. No binding to stylets of a non-vector species was observed, and a point mutation abolishing P2 transmission activity correlated with impaired stylet binding. The novel receptor appears to be a nonglycosylated protein deeply embedded in the chitin matrix. Insight into such insect receptor molecules will begin to open the major black box of this scientific field and might lead to new strategies to combat viral spread.