Properties of Glucose Sensors Prepared by the Electropolymerization of Pyrrole Containing a Gluconyl Group
Properties of Glucose Sensors Prepared by the Electropolymerization of Pyrrole Containing a Gluconyl Group
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含葡萄糖基吡咯电聚合制备的葡萄糖传感器的性能
DOI：
10.2116/analsci.15.1175
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发表时间：
1999
影响因子：
1.6
通讯作者：
T. Nakaya
中科院分区：
文献类型：
--
作者：
M. Yasuzawa;Shin;A. Kunugi;T. Nakaya
制备了GOD固定化电极。研究了该电极的葡萄糖传感器性能。GHP是根据先前报告的修改合成的。7 GOD固定化电极的制备是在4 ℃下，在铂丝电极（5 mm长，0.5mm直径）上，通过电氧化含有GHP（0.1moldm-3）、GOD（1 mgcm-3）和LiClO 4（0.1moldm-3）的脱气的0.1moldm-3磷酸盐缓冲溶液（pH7.4）来制备的。施加1.2V的恒定电势（Ag/AgCl在3.0moldm-3 KCl参比电极中，下文中的电势相对于该参比电极引用）用于电聚合;通过的沉积电荷的量为0.2Ccm-2。在pH7.4的0.1moldm-3磷酸盐缓冲溶液中，通过在0.55V电位下测量电氧化电流，考察了所制备电极对葡萄糖的安培响应（用于过氧化氢检测），除了改变施加的电势的影响被验证的实验之外，使背景电流稳定至少20 min。通过向传感器中加入增加量的葡萄糖来进行传感器的校准。
was applied to prepare a GOD-immobilized electrode. The glucose sensor properties of the obtained electrode were investigated. GHP was synthesized according to a modification of a previous report. 7 The GOD-immobilized electrode was prepared by electrooxidizing deaerated 0.1 mol dm –3 phosphate buffer solutions (pH 7.4) containing GHP (0.1 mol dm –3 ), GOD (1 mg cm –3 ) and LiClO 4 (0.1 mol dm –3 ) at 4˚C on a platinum-wire electrode (5 mm long, 0.5 mm diameter). A constant potential of 1.2 V (Ag/AgCl in 3.0 mol dm –3 KCl reference electrode, the potentials hereinafter being quoted against this reference electrode) was applied for the electropolymerization; the amount of deposition charge passed was 0.2 C cm –2 . Amperometric responses of the prepared electrodes to glucose were examined in a 0.1 mol dm –3 phosphate buffer solution of pH 7.4 by measuring the electrooxi-dation current at a potential of 0.55 V (for hydrogen peroxide detection), except for experiments where the effect of varying applied potential was being investigat-ed. The background current was allowed to stabilize for at least 20 min. Calibration of the sensor was car-ried out by adding increasing amounts of glucose to the