Intestinal myoelectric activity in response to live Vibrio cholerae and cholera enterotoxin.
Intestinal myoelectric activity in response to live Vibrio cholerae and cholera enterotoxin.
复制标题
对活霍乱弧菌和霍乱肠毒素的肠道肌电活动。
DOI：
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发表时间：
1976
影响因子：
15.9
通讯作者：
S. Cohen
中科院分区：
文献类型：
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作者：
J. Mathias;G. Carlson;A. Dimarino;G. Bertiger;H. Morton;S. Cohen
本文研究了家兔回肠对霍乱弧菌活菌培养物、霍乱全细胞裂解物和纯化肠毒素的肌电反应。每种霍乱制剂产生一系列高度组织化的迁移动作电位复合物（MAPC）。MAPC定义为持续时间为2.5 s或更长的动作电位放电，随后在至少一个其他连续电极部位发生类似活动。MAPC活性的平均值和模态发生时间4小时后，感染活霍乱弧菌培养物，冻干全细胞裂解物制剂，或纯化的肠毒素。在活动开始后，这种模式持续记录期间（长达12小时）。MAPC的平均传播速度为0.85+/-0.07 cm/s（平均值+/-SEM），随时间保持恒定。直接观察袢显示MAPC导致收缩，将管腔内内容物向外向方向推进。12 cm回肠袢的平均液体排出量为6.4+/-1.1 ml/h（平均值+/-SEM）。对照实验由以下记录组成：（a）结扎的回肠环，其中没有放置任何东西;（B）结扎的回肠环，其中注入未感染的培养液或0.9%NaCl溶液;（c）以11.2ml/h的速率用0.9%NaCl溶液灌注结扎的回肠袢，和（d）快速注射1.0，2.5，5.0，或10.0 ml 0.9% NaCl推注至近端导管。在任何对照实验中均未观察到MAPC活性。这些研究表明，除了霍乱的分泌成分外，还存在高度组织化的MAPC，其导致收缩，将管腔内的内容物向海外方向推进。
The myoelectric response of the rabbit ileum was studied in response to live Vibrio cholerae culture, a whole cell lysate of cholera, and the purified enterotoxin. Each cholera preparation produced a series of highly organized migrating action potential complexes (MAPC). An MAPC was defined as action potential discharge with a duration of 2.5 s or longer, followed by similar activity on at least one other consecutive electrode site. The mean and modal onset time of MAPC activity occurred 4 h after the infection with live Vibrio cholerae culture, the freeze-dried whole cell lysate preparation, or the purified enterotoxin. After the onset of activity this pattern persisted for the duration of the recording period (up to 12 h). The MAPC had a mean propagation velocity of 0.85+/-0.07 cm/s (mean+/-SEM), which remained constant with time. Direct visual observation of the loop revealed that the MAPC's resulted in contractions that propelled intraluminal contents in an aborad direction. The mean fluid output from the 12-cm ileal loops was 6.4+/-1.1 ml/h (mean+/-SEM). Control experiments consisted of recordings from: (a) a ligated ileal loop into which nothing was placed; (b) a ligated ileal loop into which either uninfected culture broth or 0.9% NaCl solution was injected; (c) a ligated ileal loop infused with 0.9% NaCl solution at a rate of 11.2 ml/h, and (d) rapid injection of 1.0, 2.5, 5.0, or 10.0-ml boluses of 0.9% NaCl into the proximal catheter. MAPC activity was not observed in any of the control experiments. These studies indicate that in addition to a secretory component to cholera, there exists a highly organized MAPC that results in contractions that propel intraluminal contents in an aborad direction.