A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency
A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency
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DOI：
10.1073/pnas.96.12.6609
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发表时间：
1999-06-08
影响因子：
11.1
通讯作者：
Fournier, MJ
中科院分区：
文献类型：
--
作者：
Samarsky, DA;Ferbeyre, G;Fournier, MJ
以U3小核仁RNA为载体，将锤头状核酶定位于酵母核仁。小核仁RNA：核酶的杂交体，称为“snorbozyme”，是代谢稳定的，并且在体内以接近100%的效率切割靶U3 RNA。这是报道的反式作用核酶的最有效的体内切割。该模型底物的一个关键优势是积累了稳定的、修剪过的裂解产物。这种性质允许在体内准确的动力学测量真实的切割。该系统为开发用于研究和治疗应用的有效核酶提供了新的途径。
A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a "snorbozyme," is metabolically stable and cleaves a target U3 RNA with nearly 100% efficiency in vivo. This is the most efficient in vivo cleavage reported for a trans-acting ribozyme. A key advantage of the model substrate featured is that a stable, trimmed cleavage product accumulates. This property allows accurate kinetic measurements of authentic cleavage in vivo. The system offers new avenues for developing effective ribozymes for research and therapeutic applications.