Siglec-15 promotes the migration of liver cancer cells by repressing lysosomal degradation of CD44
Siglec-15 promotes the migration of liver cancer cells by repressing lysosomal degradation of CD44
复制标题
Siglec-15 通过抑制 CD44 的溶酶体降解来促进肝癌细胞的迁移。
DOI：
10.1002/1873-3468.14169
复制
发表时间：
2021-08-11
期刊：
影响因子：
3.5
通讯作者：
Jiang, Jianhai
中科院分区：
文献类型：
--
作者：
Liu, Weitao;Ji, Zhi;Jiang, Jianhai
Sialic acid-binding immunoglobulin-like lectin-15 (Siglec-15) has been identified as a novel potential target for cancer immunotherapy. Here, we explored the role of Siglec-15 in human hepatoma cells. In this study, we found that the expression of Siglec-15 is substantially upregulated in liver cancer tissues in comparison with the nontumor tissues. Functionally, in vitro experiments show that Siglec-15 promotes the migration of hepatoma cells. Furthermore, the data demonstrated an interaction between Siglec-15 and CD44, a transmembrane glycoprotein that mediates tumor progression and metastasis. In addition, we show that CD44 is modified by alpha 2,6-linked sialic acids on N-glycans in hepatoma cells and that CD44 sialylation affects its interaction with Siglec-15. Removal of the sialic acid residues from CD44 resulted in suppressed interaction between Siglec-15 and CD44. We further demonstrate that Siglec-15 interacts and promotes the stability of CD44 by preventing its lysosomal-mediated degradation. Taken together, our findings demonstrate that Siglec-15 promotes the migration of hepatoma cells by regulating the CD44 protein stability.
