Racial tropism of a highly toxic clone of Actinobacillus actinomycetemcomitans associated with juvenile periodontitis
Racial tropism of a highly toxic clone of Actinobacillus actinomycetemcomitans associated with juvenile periodontitis
复制标题
DOI：
10.1128/jcm.35.12.3037-3042.1997
复制
发表时间：
1997-12-01
影响因子：
9.4
通讯作者：
Kilian, M
中科院分区：
文献类型：
--
作者：
Haubek, D;Dirienzo, JM;Kilian, M
伴随放线放线杆菌菌株，具有增强的白细胞毒素产生水平，其特征在于白细胞毒素基因操纵子的启动子区域的530-bp缺失。以前的分离株与此缺失构成了一个单一的克隆属于血清型B，虽然他们之间显示出微小的差异。我们通过对326 A.伴放线菌分离自健康和牙周病个体以及来自世界各国的患有不同类型口腔外感染的患者。共有38个菌株，都属于同一克隆，显示530-bp的缺失。从这些菌株中的10个白细胞毒素基因操纵子的启动子区域的440 bp序列的比较揭示了完全的同一性，这表明缺失起源于一个单一的突变事件。这个特殊的克隆是专门与局限性青少年牙周炎（LJP）。在28个分离出克隆的家庭中，至少有12个家庭中有一名以上的家庭成员患有LJP。值得注意的是，所有携带这个克隆体的受试者都与非洲人口有遗传联系。这些观察结果表明，青少年牙周炎在一些青少年与非洲起源与传播的克隆A。伴放线菌
Actinobacillus actinomycetemcomitans strains,vith enhanced levels of production of leukotoxin are characterized by a 530-bp deletion from the promoter region of the leukotoxin gene operon. Previous isolates with this deletion constituted a single clone belonging to serotype b, although they displayed minor differences among each other. We have analyzed the geographic dissemination of this clone by examining 326 A. actinomycetemcomitans isolates from healthy and periodontally diseased individuals as well as from patients with different types of extraoral infections originating from countries worldwide. A total of 38 isolates, all belonging to the same clone, showed the 530-bp deletion. Comparison of a 440-bp sequence from the promoter region of the leukotoxin gene operon from 10 of these strains revealed complete identity, which indicates that the deletion originates from a single mutational event. This particular clone was exclusively associated with localized juvenile periodontitis (LJP). In at least 12 of 28 families from which the clone was isolated, more than one family member had LJP. Notably, all the subjects carrying this clone had a genetic affiliation with the African population. These observations suggest that juvenile periodontitis in some adolescents with an African origin is associated with a disseminating clone of A. actinomycetemcomitans.