Orthotopic mouse model of colorectal cancer.
Orthotopic mouse model of colorectal cancer.
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DOI：
10.3791/484
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Engleman, Edgar
中科院分区：
文献类型：
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作者：
Tseng, William;Leong, Xianne;Engleman, Edgar
传统的皮下肿瘤模型对于研究结直肠癌并不理想。结肠直肠癌的原位小鼠模型，其特征是癌细胞在其自然位置生长，以高保真度复制人类疾病。可以使用两种技术来建立这个模型。这两种技术是相似的，需要小鼠麻醉和剖腹手术暴露盲肠。一种技术涉及将结肠直肠癌细胞悬液注射到盲肠壁中。癌细胞首先在培养物中生长，在亚融合时收获并制备成单细胞悬浮液。缓慢注射少量细胞以避免泄漏。另一种技术是将一块皮下肿瘤移植到盲肠上。将具有先前建立的皮下结肠直肠肿瘤的小鼠安乐死，并使用无菌技术去除肿瘤。将肿瘤块分成小块用于移植到另一只小鼠。在移植之前，盲肠壁被轻微损伤以促进肿瘤细胞浸润。发展原发性肿瘤和肝转移的时间将取决于所使用的技术、细胞系和小鼠物种。这种原位小鼠模型可用于研究结直肠癌的自然进展和测试针对结直肠癌的新治疗剂。
The traditional subcutaneous tumor model is less than ideal for studying colorectal cancer. Orthotopic mouse models of colorectal cancer, which feature cancer cells growing in their natural location, replicate human disease with high fidelity. Two techniques can be used to establish this model. Both techniques are similar and require mouse anesthesia and laparotomy for exposure of the cecum. One technique involves injection of a colorectal cancer cell suspension into the cecal wall. Cancer cells are first grown in culture, harvested when subconfluent and prepared as a single cell suspension. A small volume of cells is injected slowly to avoid leakage. The other technique involves transplantation of a piece of subcutaneous tumor onto the cecum. A mouse with a previously established subcutaneous colorectal tumor is euthanized and the tumor is removed using sterile technique. The tumor piece is divided into small pieces for transplantation to another mouse. Prior to transplantation, the cecal wall is lightly damaged to facilitate tumor cell infiltration. The time to developing primary tumors and liver metastases will vary depending on the technique, cell line, and mouse species used. This orthotopic mouse model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.