In vitro propagation of Japanese garden iris, Iris ensata Thunb.
In vitro propagation of Japanese garden iris, Iris ensata Thunb.
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日本花园鸢尾 (Iris ensata Thunb) 的体外繁殖。
DOI：
10.1007/bf00040480
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发表时间：
1991
期刊：
影响因子：
1.9
通讯作者：
T. Adachi
中科院分区：
文献类型：
--
作者：
T. Yabuya;Y. Ikeda;T. Adachi
以马蔺（Iris ensata）幼嫩花葶为外植体，在MS + NAA 1 mg/l +6-BA 1 mg/l+蔗糖30 g/l+琼脂10 g/l的培养基上进行愈伤组织、芽和根的诱导。在23个品种和一个野生型中，“Okichidori”，“Miyukisudare”和“Meiji-l”表现出相当大的芽诱导率，虽然这些诱导生根差的芽。此外，观察到绿色和白色两种类型的愈伤组织的诱导，并且绿色型愈伤组织的诱导与芽的诱导显著相关。令人惊讶的是，对上述培养基的唯一修改，半强度MS无机盐，被证明是非常有效的芽诱导在花茎培养。以1/2 MS + NAA 1 mg/l +6-BA 1 mg/l+蔗糖30 g/l+琼脂10 g/l为基本培养基，研究了蔗糖浓度和活性炭对花茎培养芽生根的影响。在培养基中添加1%活性炭对根的诱导有显著的效果，与蔗糖浓度和供试品种无关。采用离体繁殖技术，Ensatais讨论。
Explants of young scapes ofIris ensatawere cultured on MS medium with 1 mg/l NAA, 1 mg/l 6-BA, 30 g/l sucrose and 10 g/l agar, and this species was characterized by high variety specificity for callus, shoot and root induction. Among 23 varieties and one wild form tested, ‘Okichidori’, ‘Miyukisudare’ and ‘Meiji-l’ exhibited a considerable rate of shoot induction, although these induced poorly rooted shoots. In addition, two types of callus induction such as green and white calli were observed, and the induction of green-type calli was significantly correlated with that of shoots. Surprisingly, the only modification, half-strength MS inorganic salts, for the above medium proved to be very effective for shoot induction in the scape culture. For shoots obtained from the scape culture, effects of sucrose concentrations and activated charcoal on root induction were examined by using 1/2 MS with 1 mg/l NAA, 1 mg/l 6-BA, 30 g/l sucrose and 10 g/l agar as the basic medium. The addition of 1% activated charcoal to the media had a marked effect for root induction independent of sucrose concentrations and varieties tested. Thein vitropropagation technique ofI. ensatais discussed.