DIVERSITY OF SEED STORAGE PROTEIN-PATTERNS IN WILD PEANUT (ARACHIS, FABACEAE) SPECIES
DIVERSITY OF SEED STORAGE PROTEIN-PATTERNS IN WILD PEANUT (ARACHIS, FABACEAE) SPECIES
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DOI：
10.1007/bf00937710
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发表时间：
1993-01-01
影响因子：
1.9
通讯作者：
MURPHY, JP
中科院分区：
文献类型：
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作者：
BIANCHIHALL, CM;KEYS, RD;MURPHY, JP
对55份野生花生（Arachis spp.）采用SDS-PAGE电泳分析了从南美引进的10种大豆种子贮藏蛋白的组成。这项研究的目的是评估组内的变异性。花生和分类类群的基础上蛋白质组成。解析了25个不同的条带位置。单个材料有11 ~ 18条带，其中包括conarachin区（分子量> 50 kD），酸性花生蛋白区有2 - 5条带（MW 38-49.9 kD），3 - 7个在中等MW区域（23 - 37.9 kD），在基础花生蛋白区（18-22.9 kD）有2 - 5条带，在低分子量蛋白区（14-17.9 kD）有1 - 3条带。这些数据被用于主坐标分析的基础上的所有对的55个加入之间的遗传距离矩阵。几组材料符合预期的种分类，包括A。batizocoi、黑腹拟杆菌A. stenosperma和A. monticola;而A. duranensis、黑腹滨藜A. cardenasii、红腹滨藜A. helodes和A.科伦蒂纳没有形成好的团队。研究表明，蛋白质谱存在很大的多样性，种子贮藏蛋白具有潜在的帮助物种分类和作为种间杂交研究的标记。
55 accessions of wild peanuts (Arachis spp.) introduced from South America were analyzed for seed storage protein composition using SDS-PAGE electrophoresis. The objectives of the study were to evaluate variability within sect. Arachis and to classify taxa based on protein composition. 25 different band positions were resolved. Individual accessions had 11 to 18 bands which included the conarachin region (MW > 50 kD), two to five bands in the acidic arachin region (MW 38-49.9 kD), three to seven in the intermediate MW region (23 to 37.9 kD), two to five bands in the basic arachin region (18-22.9 kD), and one to three bands in the low MW protein region (14-17.9 kD). These data were utilized in a principal coordinate analysis based on the matrix of genetic distances between all pairs of the 55 accessions. Several groups of accessions conformed to expected species classification including A. batizocoi, A. stenosperma, and A. monticola; while A. duranensis, A. cardenasii, A. helodes, and A. correntina did not form good groups. The study showed that great diversity exists for protein profiles and seed storage proteins have potential for aiding species classification and for serving as markers for interspecific hybridization studies.