Genome-wide association studies for fatty acid metabolic traits in five divergent pig populations.
Genome-wide association studies for fatty acid metabolic traits in five divergent pig populations.
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五个不同猪群脂肪酸代谢特征的全基因组关联研究
DOI：
10.1038/srep24718
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发表时间：
2016-04-21
影响因子：
4.6
通讯作者：
Huang L
中科院分区：
文献类型：
--
作者：
Zhang W;Bin Yang;Zhang J;Cui L;Ma J;Chen C;Ai H;Xiao S;Ren J;Huang L
脂肪酸组成是肉品质和风味的重要指标。脂肪酸代谢指标更能真实反映肉类营养状况和公众接受程度。为探讨猪肉脂肪酸代谢指标的遗传机制，对5个猪群体的33个脂肪酸代谢性状进行了全基因组关联分析。我们共鉴定了865个单核苷酸多态性（SNP），对应于9条染色体上的11个全基因组显著位点和9条染色体上的12个提示位点。我们的研究结果不仅证实了7个先前报道的具有较强关联强度的QTL，而且还揭示了4个新的群体特异性位点，表明对脂肪酸代谢性状等中间表型的研究可以增加GWAS对终点表型的统计功效。我们提出了一个候选基因的名单，在所确定的位点，包括三个新的基因（FADS 2，SREBF 1和PLA2G7）。在此基础上，我们构建了这些候选基因的功能网络，并推测了潜在的脂肪酸代谢途径。这些发现推进了我们对猪脂肪酸组成的遗传基础的理解。欧洲杂交商品猪的结果可以立即转化为有益的脂肪酸组成的育种实践。
Fatty acid composition profiles are important indicators of meat quality and tasting flavor. Metabolic indices of fatty acids are more authentic to reflect meat nutrition and public acceptance. To investigate the genetic mechanism of fatty acid metabolic indices in pork, we conducted genome-wide association studies (GWAS) for 33 fatty acid metabolic traits in five pig populations. We identified a total of 865 single nucleotide polymorphisms (SNPs), corresponding to 11 genome-wide significant loci on nine chromosomes and 12 suggestive loci on nine chromosomes. Our findings not only confirmed seven previously reported QTL with stronger association strength, but also revealed four novel population-specific loci, showing that investigations on intermediate phenotypes like the metabolic traits of fatty acids can increase the statistical power of GWAS for end-point phenotypes. We proposed a list of candidate genes at the identified loci, including three novel genes (FADS2, SREBF1 and PLA2G7). Further, we constructed the functional networks involving these candidate genes and deduced the potential fatty acid metabolic pathway. These findings advance our understanding of the genetic basis of fatty acid composition in pigs. The results from European hybrid commercial pigs can be immediately transited into breeding practice for beneficial fatty acid composition.