Available surface dictates microbial adhesion capacity
Available surface dictates microbial adhesion capacity
复制标题
DOI：
10.1016/j.ijadhadh.2014.01.027
复制
发表时间：
2014-04-01
影响因子：
3.4
通讯作者：
Raspor, Peter
中科院分区：
文献类型：
--
作者：
Bohinc, Klemen;Drazic, Goran;Raspor, Peter
细菌粘附可以通过不同的材料表面特性（如表面粗糙度）来控制（抑制或促进），这是我们研究的重点。通过抛光具有不同梯度的玻璃板来制备四种不同的玻璃表面。相应的表面粗糙度由原子力显微镜和轮廓仪控制。在实验中，我们使用了一种革兰氏阳性菌（金黄色葡萄球菌）和两种革兰氏阴性菌（铜绿假单胞菌和大肠杆菌）。用比色法测定玻璃表面细菌的粘附率。结果表明，表面粗糙度越大，细菌粘附率越高。细菌在更粗糙表面上的粘附增加是有效表面增加和表面上缺陷数量增加之间的相互作用。为了控制所有参数，我们还测量了细菌和玻璃表面的表面电荷密度和疏水性。(C)2014爱思唯尔有限公司版权所有。
The bacterial adhesion can be controlled (inhibit or promote) by different material surface characteristics like surface roughness, on which we concentrate in our study. Four different glass surfaces were prepared by polishing the glass plates with different gradations. The corresponding surface roughness was controlled by atomic force microscope and profilometer. For experiments we have used one Gram-positive bacterium (Staphylococcus aureus) and two Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli). The rate of adhered bacteria on glass surfaces was determined spectrophotometrically. The results showed that the rate of adhered bacteria increases with increasing surface roughness. The increased adhesion of bacteria on more rough surfaces is the interplay between the increasing effective surface and increasing number of defects on the surface. In order to keep all parameters under control we have also measured the surface charge density and hydrophobicity of bacteria and glass surfaces as well. (C) 2014 Elsevier Ltd. All rights reserved.