Focusing light in biological tissue through a multimode optical fiber: refractive index matching.
Focusing light in biological tissue through a multimode optical fiber: refractive index matching.
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DOI：
10.1364/ol.44.002386
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发表时间：
2019-05
期刊：
影响因子：
3.6
通讯作者：
Raphaël Turcotte;Carla C. Schmidt;N. Emptage;M. Booth
中科院分区：
文献类型：
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作者：
Raphaël Turcotte;Carla C. Schmidt;N. Emptage;M. Booth
通过阶跃折射率多模光纤(MMF)控制光的传输有几个重要的应用，包括生物成像。然而，很少考虑光纤和组织光学的耦合。在这封信中，我们描述了组织诱导的光扭曲的影响，特别是那些由成像前校准和生物介质的折射率不匹配引起的影响。通过在与大脑折射率匹配的介质中进行校准，实现了最佳的对焦能力，以及视场内对焦均匀度的增加。这些照明变化导致珠子和活脑组织的荧光图像的空间分辨率和强度提高了30%。除了折射率匹配，我们的结果表明，样本诱导的像差可以严重恶化基于MMF的系统的图像。
Controlling light propagation through a step-index multimode optical fiber (MMF) has several important applications, including biological imaging. However, little consideration has been given to the coupling of fiber and tissue optics. In this Letter, we characterized the effects of tissue-induced light distortions, in particular those arising from a mismatch in the refractive index of the pre-imaging calibration and biological media. By performing the calibration in a medium matching the refractive index of the brain, optimal focusing ability was achieved, as well as a gain in focus uniformity within the field-of-view. These changes in illumination resulted in a 30% improvement in spatial resolution and intensity in fluorescence images of beads and live brain tissue. Beyond refractive index matching, our results demonstrate that sample-induced aberrations can severely deteriorate images from MMF-based systems.