Distribution of β-glucosidase and β-glucuronidase activity and of β-glucuronidase gene gus in human colonic bacteria
Distribution of β-glucosidase and β-glucuronidase activity and of β-glucuronidase gene gus in human colonic bacteria
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DOI：
10.1111/j.1574-6941.2008.00520.x
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发表时间：
2008-12-01
影响因子：
4.2
通讯作者：
Louis, Petra
中科院分区：
文献类型：
--
作者：
Dabek, Marta;McCrae, Sheila I.;Louis, Petra
存在于人类结肠微生物群中的β-糖苷酶活性作用于进入结肠的糖苷植物次级化合物和异生物质，对人类宿主具有潜在的健康影响。关于β-糖苷酶的信息目前仅限于来自人类结肠生态系统的相对较少的细菌物种。因此，我们筛选了40种不同的细菌菌株，这些菌株代表了来自人类粪便的β-葡萄糖苷酶和β-葡萄糖醛酸酶活性的优势细菌群。超过一半的低G+C%革兰氏阳性厚壁菌门具有β-葡萄糖苷酶活性，而β-葡萄糖醛酸酶活性仅在梭菌簇XIVa和IV内的一些厚壁菌门中发现。大多数双歧杆菌属（Bifidobacterium spp.）多形拟杆菌具有β-葡萄糖苷酶活性。基于简并PCR，在40株分离株中的10株中检测到属于第2家族糖基水解酶的β-葡萄糖醛酸酶基因。这些包括所有9个分离株，β-葡萄糖醛酸酶活性检测呈阳性，这表明简并PCR可以提供一个有用的测定肠道群落中产生β-葡萄糖醛酸酶的能力。在Roseburia hominis A2-183中，β-葡萄糖醛酸酶活性通过在d-葡萄糖醛酸上生长或通过添加4-硝基苯酚-葡萄糖醛酸苷诱导，而β-葡萄糖苷酶活性通过4-硝基苯酚-吡喃葡萄糖苷诱导。不同菌株之间的诱导性不同。
beta-Glycosidase activities present in the human colonic microbiota act on glycosidic plant secondary compounds and xenobiotics entering the colon, with potential health implications for the human host. Information on beta-glycosidases is currently limited to relatively few species of bacteria from the human colonic ecosystem. We therefore screened 40 different bacterial strains that are representative of dominant bacterial groups from human faeces for beta-glucosidase and beta-glucuronidase activity. More than half of the low G+C% Gram-positive firmicutes harboured beta-glucosidase activity, while beta-glucuronidase activity was only found in some firmicutes within clostridial clusters XIVa and IV. Most of the Bifidobacterium spp. and Bacteroides thetaiotaomicron carried beta-glucosidase activity. A beta-glucuronidase gene belonging to family 2 glycosyl hydrolases was detected in 10 of the 40 isolates based on degenerate PCR. These included all nine isolates that gave positive assays for beta-glucuronidase activity, suggesting that the degenerate PCR could provide a useful assay for the capacity to produce beta-glucuronidase in the gut community. beta-Glucuronidase activity was induced by growth on d-glucuronic acid, or by addition of 4-nitrophenol-glucuronide, in Roseburia hominis A2-183, while beta-glucosidase activity was induced by 4-nitrophenol-glucopyranoside. Inducibility varied between strains.