Mammalian cell transfection: the present and the future.

Mammalian cell transfection: the present and the future.
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DOI:
10.1007/s00216-010-3821-6
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发表时间:
2010-08
影响因子:
4.3
通讯作者:
Eberwine, James H.
Eberwine, James H.
中科院分区:
化学2区
文献类型:
--
作者:
Kim, Tae Kyung;Eberwine, James H.

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转染是一种强大的分析工具,可以研究细胞中基因和基因产物的功能。转染方法大致分为三大类;生物的、化学的和物理的。这些方法已经发展到可以通过使用精确控制的激光显微镜系统将核酸输送到细胞的特定亚细胞区域。点定向转染与mRNA转染相结合是研究基因和基因产物功能的新途径。然而,每种方法都有自己的优点和缺点,因此最优方法取决于实验设计和目的。
Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microcope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.
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