Mammalian cell transfection: the present and the future.
Mammalian cell transfection: the present and the future.
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DOI:
10.1007/s00216-010-3821-6
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发表时间:
2010-08
影响因子:
4.3
通讯作者:
Eberwine, James H.
中科院分区:
文献类型:
--
作者:
Kim, Tae Kyung;Eberwine, James H.
Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical. These methods have advanced to make it possible to deliver nucleic acids to specific subcellular regions of cells by use of a precisely controlled laser-microcope system. The combination of point-directed transfection and mRNA transfection is a new way of studying the function of genes and gene products. However, each method has its own advantages and disadvantages so the optimum method depends on experimental design and objective.
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发表时间:
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