IDENTIFICATION OF AN EARLY-GROWTH-RESPONSE GENE ENCODING A NOVEL PUTATIVE PROTEIN-KINASE
IDENTIFICATION OF AN EARLY-GROWTH-RESPONSE GENE ENCODING A NOVEL PUTATIVE PROTEIN-KINASE
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DOI：
10.1128/mcb.12.9.4164
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发表时间：
1992-09-01
影响因子：
5.3
通讯作者：
ERIKSON, RL
中科院分区：
文献类型：
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作者：
SIMMONS, DL;NEEL, BG;ERIKSON, RL
早期生长反应基因，也称为立即早期基因，在调节细胞增殖中起重要作用。我们已经确定了一种新型的早期生长反应基因产物，一个77，811-Da推定的丝氨酸/苏氨酸激酶，这是高度诱导的血清和佛波酯。编码这种推定激酶的mRNA在用有丝分裂原处理后1小时内显著升高，并且这种诱导通过放线菌酮协同增加。地塞米松阻断激酶mRNA的血清诱导，正如v-Ki-ras的转化一样。在小鼠脑、肺和心脏中检测到激酶mRNA。这种新的假定激酶，我们称之为Snk，血清诱导激酶，在其拟议的催化结构域中与许多其他蛋白激酶相似;然而，没有其他激酶与Snk显示出足够的序列相似性，表明存在共同的功能。因此，Snk代表了一种新型的蛋白激酶，参与早期促有丝分裂反应，其活性受到转录和转录后调节。
Early-growth-response genes, also known as immediate-early genes, play important roles in regulating cell proliferation. We have identified a new type of early-growth-response gene product, a 77,811-Da putative serine/threonine kinase, which is highly inducible by serum and phorbol ester. mRNA encoding this putative kinase is markedly elevated within 1 h after treatment with mitogen, and this induction is synergistically increased by cycloheximide. Dexamethasone blocks serum induction of the kinase mRNA, as does transformation by v-Ki-ras. The kinase mRNA was detected in mouse brain, lung, and heart. This new putative kinase, which we term Snk, for serum-inducible kinase, showed similarity in its proposed catalytic domain to many other protein kinases; however, no other kinase showed enough sequence similarity with Snk to suggest the existence of a common function. Hence, Snk represents a new type of protein kinase involved in the early mitogenic response whose activity is transcriptionally and posttranscriptionally regulated.