Influence of alterations in acid-base conditions on intracellular pH of intact renal cortex.
Influence of alterations in acid-base conditions on intracellular pH of intact renal cortex.
复制标题
酸碱条件的变化对完整肾皮质细胞内 pH 值的影响。
DOI：
10.1159/000172878
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Simpson,DP
中科院分区：
文献类型：
--
作者：
Jones,B;Simpson,DP
在代谢性酸中毒、钾耗竭和碳酸酐酶抑制条件下，用[14 C]-5，5-二甲基-2，4-恶唑烷二酮和22 Na+测定了完整大鼠肾皮质细胞内pH值（pHI）。在代谢性酸中毒和碱中毒中，pH值和碳酸氢盐浓度的变化方向与血浆中的变化方向相同。在钾耗竭中，全身酸碱平衡未改变，但发生了明显的细胞内酸中毒。乙酰唑胺对碳酸酐酶的抑制作用与细胞外呼吸性酸中毒和细胞内碳酸氢盐浓度升高有关。另一种碳酸酐酶抑制剂，苯甲酰胺，引起全身酸碱状态没有变化，但产生细胞内碳酸氢盐浓度的降低。当对肾小管液碳酸氢盐和细胞内钠的预测变化进行适当校正时，上述变化的绝对值发生了变化，但pH值和碳酸氢盐浓度变化的方向没有改变。
Intracellular pH (pHI) of intact rat renal cortex was estimated using [14C]-5,5-dimethyl-2,4-oxazolidinedione and22Na+under conditions of metabolic acidosis and alkalosis, potassium depletion and carbonic anhydrase inhibition. In metabolic acidosis and alkolosis, pHIand bicarbonate concentration changed in the same direction as occurred in plasma. In potassium depletion, systemic acid-base balance was unaltered but a marked intracellular acidosis developed. Carbonic anhydrase inhibition with acetazolamide was associated with an extracellular respiratory acidosis and a rise in intracellular bicarbonate concentration. Another carbonic anhydrase inhibitor, benzolamide, caused no change in systemic acid-base state but produced a decrease in intracellular bicarbonate concentration. When appropriate corrections were made for predicted change in tubular fluid bicarbonate and for intracellular sodium, modification in the absolute values of the above changes occurred but the directions of the changes in pHIand bicarbonate concentration were unaltered.