Preservation of the bone protein osteocalcin in dinosaurs
Preservation of the bone protein osteocalcin in dinosaurs
复制标题
恐龙骨蛋白骨钙素的保存
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
P. Westbroek
中科院分区：
文献类型：
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作者：
G. Muyzer;P. Sandberg;M. Knapen;C. Vermeer;M. Collins;P. Westbroek
两种不同的免疫学检测方法被用于鉴定恐龙和其他脊椎动物化石骨骼中骨基质蛋白骨钙素（OC）的残留物。从现代脊椎动物的抗OC抗体表现出强烈的免疫交叉反应与现代和相对年轻的化石样品和显着的反应与一些恐龙骨提取物。OC的存在通过检测肽结合的脊椎动物特有的氨基酸γ羧基谷氨酸（Gla）来证实。骨化石中OC的保存似乎强烈依赖于埋葬历史，而不仅仅是年龄。这些结果扩大了蛋白质在地质记录中的保存范围，并为恐龙的分子进化迈出了第一步。
Two different immunological assays were used to identify the remains of a bone matrix protein, osteocalcin (OC), in the bones of dinosaurs and other fossil vertebrates. Antibodies raised against OC from modern vertebrates showed strong immunological cross-reactivity with modern and relatively young fossil samples and significant reactions with some of the dinosaur bone extracts. The presence of OC was confirmed by the detection of a peptide-bound, uniquely vertebrate amino acid, γcarboxyglutamic acid (Gla). Preservation of OC in fossil bones appears to be strongly dependent on the burial history and not simply on age. These results extend the range of protein preservation in the geologic record and provide a first step toward a molecular phylogeny of the dinosaurs.